Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :12819204
Publication Date : //

Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends.


Messenger RNA 3'-end formation is functionally coupled to transcription by RNA polymerase II. By tagging and purifying Ref2, a non-essential protein previously implicated in mRNA cleavage and termination, we isolated a multiprotein complex, holo-CPF, containing the yeast cleavage and polyadenylation factor (CPF) and six additional polypeptides. The latter can form a distinct complex, APT, in which Pti1, Swd2, a type I protein phosphatase (Glc7), Ssu72 (a TFIIB and RNA polymerase II-associated factor), Ref2, and Syc1 are associated with the Pta1 subunit of CPF. Systematic tagging and purification of holo-CPF subunits revealed that yeast extracts contain similar amounts of CPF and holo-CPF. By purifying holo-CPF from strains lacking Ref2 or containing truncated subunits, subcomplexes were isolated that revealed additional aspects of the architecture of APT and holo-CPF. Chromatin immunoprecipitation was used to localize Ref2, Ssu72, Pta1, and other APT subunits on small nucleolar RNA (snoRNA) genes and primarily near the polyadenylation signals of the constitutively expressed PYK1 and PMA1 genes. Use of mutant components of APT revealed that Ssu72 is important for preventing readthrough-dependent expression of downstream genes for both snoRNAs and polyadenylated transcripts. Ref2 and Pta1 similarly affect at least one snoRNA transcript.

Authors : Nedea Eduard , He Xiaoyuan , Kim Minkyu , Pootoolal Jeff , Zhong Guoqing , Canadien Veronica , Hughes Timothy , Buratowski Stephen , Moore Claire L , Greenblatt Jack ,

Related products :

Catalog number Product name Quantity
29-473 FIP1L1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting w 0.05 mg
29-435 CPSF2 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting wit 0.05 mg
29-411 CPSF1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting wi 0.05 mg
EIAAB09127 CFIm59,Cleavage and polyadenylation specificity factor 59 kDa subunit,Cleavage and polyadenylation specificity factor subunit 7,CPSF 59 kDa subunit,CPSF7,Homo sapiens,Human,Pre-mRNA cleavage factor Im
20-372-60192 cleavage and polyadenylation specific factor 4. 30kDa (CPSF4). mRNA - Mouse monoclonal anti-human CPSF4 antibody; Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; N 0.1 mg
EIAAB09123 CFIm68,CFIM68,Cleavage and polyadenylation specificity factor 68 kDa subunit,Cleavage and polyadenylation specificity factor subunit 6,CPSF 68 kDa subunit,CPSF6,Homo sapiens,Human,Pre-mRNA cleavage fa
EIAAB09112 Cleavage and polyadenylation specificity factor 73 kDa subunit,Cleavage and polyadenylation specificity factor subunit 3,CPSF 73 kDa subunit,CPSF3,CPSF73,Homo sapiens,Human,mRNA 3'-end-processing endo
EIAAB09110 Bos taurus,Bovine,Cleavage and polyadenylation specificity factor 73 kDa subunit,Cleavage and polyadenylation specificity factor subunit 3,CPSF 73 kDa subunit,CPSF3,CPSF73,mRNA 3'-end-processing endon
18-003-43626 Cleavage and polyadenylation specificity factor subunit 5 - Cleavage and polyadenylation specificity factor 25 kDa subunit; CPSF 25 kDa subunit; Pre-mRNA cleavage factor Im 25 kDa subunit; Nucleoside 0.1 mg Protein A
18-003-43657 Cleavage and polyadenylation specificity factor subunit 7 - Cleavage and polyadenylation specificity factor 59 kDa subunit; CPSF 59 kDa subunit; Pre-mRNA cleavage factor Im 59 kDa subunit Polyclonal 0.1 mg Protein A
18-003-43627 Cleavage and polyadenylation specificity factor subunit 6 - Cleavage and polyadenylation specificity factor 68 kDa subunit; CPSF 68 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPB 0.1 mg Protein A
EIAAB09111 Cleavage and polyadenylation specificity factor 73 kDa subunit,Cleavage and polyadenylation specificity factor subunit 3,CPSF 73 kDa subunit,Cpsf3,Cpsf73,Mouse,mRNA 3'-end-processing endonuclease CPSF
25-603 CSTF3 is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 0.05 mg
29-270 CSTF3 is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 0.05 mg
60192 IgG,cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), mRNA 0.1 mg
EIAAB09115 Cleavage and polyadenylation specificity factor 30 kDa subunit,Cleavage and polyadenylation specificity factor subunit 4,CPSF 30 kDa subunit,CPSF30,CPSF4,Homo sapiens,Human,NAR,NEB1,Neb-1,No arches ho
EIAAB09116 Cleavage and polyadenylation specificity factor 30 kDa subunit,Cleavage and polyadenylation specificity factor subunit 4,Clipper homolog,Clipper_CPSF 30K,CPSF 30 kDa subunit,Cpsf30,Cpsf4,Mouse,Mus mus
EIAAB09107 Cleavage and polyadenylation specificity factor 100 kDa subunit,Cleavage and polyadenylation specificity factor subunit 2,CPSF 100 kDa subunit,CPSF100,CPSF2,Homo sapiens,Human,KIAA1367
EIAAB09109 Cleavage and polyadenylation specificity factor 100 kDa subunit,Cleavage and polyadenylation specificity factor subunit 2,CPSF 100 kDa subunit,Cpsf100,Cpsf2,Mcpsf,Mouse,Mus musculus
EIAAB09106 Cleavage and polyadenylation specificity factor 160 kDa subunit,Cleavage and polyadenylation specificity factor subunit 1,CPSF 160 kDa subunit,CPSF1,CPSF160,Homo sapiens,Human
18-003-44209 Cleavage and polyadenylation specificity factor subunit 4 - Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches 0.05 mg Aff Pur
EIAAB09114 Bos taurus,Bovine,Cleavage and polyadenylation specificity factor 30 kDa subunit,Cleavage and polyadenylation specificity factor subunit 4,CPSF 30 kDa subunit,CPSF30,CPSF4
EIAAB09105 Bos taurus,Bovine,Cleavage and polyadenylation specificity factor 160 kDa subunit,Cleavage and polyadenylation specificity factor subunit 1,CPSF 160 kDa subunit,CPSF1,CPSF160
EIAAB09104 Cleavage and polyadenylation specificity factor 160 kDa subunit,Cleavage and polyadenylation specificity factor subunit 1,CPSF 160 kDa subunit,Cpsf1,Cpsf160,Mouse,Mus musculus
EIAAB09108 Bos taurus,Bovine,Cleavage and polyadenylation specificity factor 100 kDa subunit,Cleavage and polyadenylation specificity factor subunit 2,CPSF 100 kDa subunit,CPSF100,CPSF2


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur