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Pubmed ID :15461802
Publication Date : //

A genome annotation-driven approach to cloning the human ORFeome.


We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

Authors : Collins John E , Wright Charmain L , Edwards Carol A , Davis Matthew P , Grinham James A , Cole Charlotte G , Goward Melanie E , Aguado Begoña , Mallya Meera , Mokrab Younes , Huckle Elizabeth J , Beare David M , Dunham Ian ,

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