Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :16641086
Publication Date : //

An expanded WW domain recognition motif revealed by the interaction between Smad7 and the E3 ubiquitin ligase Smurf2.

Smurf2 is an E3 ubiquitin ligase that drives degradation of the transforming growth factor-beta receptors and other targets. Recognition of the receptors by Smurf2 is accomplished through an intermediary protein, Smad7. Here we have demonstrated that the WW3 domain of Smurf2 can directly bind to the Smad7 polyproline-tyrosine (PY) motif. Of particular interest, the highly conserved WW domain binding site Trp, which interacts with target PY motifs, is a Phe in the Smurf2 WW3 domain. To examine this interaction, the solution structure of the complex between the Smad7 PY motif region (ELESPPPPYSRYPMD) and the Smurf2 WW3 domain was determined. The structure reveals that, in addition to binding the PY motif, the WW3 domain binds six residues C-terminal to the PY motif (PY-tail). Although the Phe in the WW3 domain binding site decreases affinity relative to the canonical Trp, this is balanced by additional interactions between the PY-tail and the beta1-strand and beta1-beta2 loop of the WW3 domain. The interaction between the Smurf2 WW3 domain and the Smad7 PY motif is the first example of PY motif recognition by a WW domain with a Phe substituted for the binding site Trp. This unusual interaction allows the Smurf2 WW3 domain to recognize a subset of PY motif-containing proteins utilizing an expanded surface to provide specificity.

Authors : Chong P Andrew , Lin Hong , Wrana Jeffrey L , Forman-Kay Julie D ,

Related products :

Catalog number Product name Quantity
EIAAB38891 E3 ubiquitin-protein ligase SMURF2,Homo sapiens,hSMURF2,Human,SMAD ubiquitination regulatory factor 2,SMAD-specific E3 ubiquitin-protein ligase 2,SMURF2
EIAAB38892 E3 ubiquitin-protein ligase SMURF2,Mouse,Mus musculus,SMAD ubiquitination regulatory factor 2,SMAD-specific E3 ubiquitin-protein ligase 2,Smurf2
CSB-EL021861MO Mouse E3 ubiquitin-protein ligase SMURF2(SMURF2) ELISA kit SpeciesMouse 96T
CSB-EL021861HU Human E3 ubiquitin-protein ligase SMURF2(SMURF2) ELISA kit SpeciesHuman 96T
CSB-EL021861HU Human E3 ubiquitin-protein ligase SMURF2(SMURF2) ELISA kit 96T
CSB-EL021861MO Mouse E3 ubiquitin-protein ligase SMURF2(SMURF2) ELISA kit 96T
SMURF2 SMURF2 Gene SMAD specific E3 ubiquitin protein ligase 2
SMUF2_MOUSE ELISA Kit FOR E3 ubiquitin-protein ligase SMURF2; organism: Mouse; gene name: Smurf2 96T
80403 Ubiquitin E3 ligase SMURF2 20 µg
OBT1362 SMURF2, E3 Ubiquitin Ligase, ~ 80, 50 & 40kD, Goat anti_Human; WB 100 µg.
25-830 FBXO21 contains 1 F-box domain. It is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.This gene encodes a member of the F-box protein family whi 0.05 mg
25-845 HACE1 contains 6 ANK repeats and 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. HACE1 is an E3 ubiquitin-protein ligase that may function in cellular proteins degradation. 0.05 mg
CSB-EL021861HU Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL021861MO Mouse SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) ELISA kit, Species Mouse, Sample Type serum, plasma 96T
25-013 RBM6 contains 1 G-patch domain and 1 RRM (RNA recognition motif) domain.It specifically binds poly (G) RNA homopolymers in vitro. 0.05 mg
E3045h Human Ubiquitin Interaction Motif Containing Prote 96T
201-20-6255 UIMC1{ubiquitin interaction motif containing 1}rabbit.pAb 0.2ml
EIAAB45119 E3 ubiquitin-protein ligase UBR5,E3 ubiquitin-protein ligase, HECT domain-containing 1,Edd,Edd1,Hyperplastic discs protein homolog,Kiaa0896,Mouse,Mus musculus,Ubr5
EIAAB25831 C1orf166,E3 ubiquitin-protein ligase MUL1,GIDE,Growth inhibition and death E3 ligase,Homo sapiens,Human,MAPL,Mitochondrial ubiquitin ligase activator of NFKB 1,Mitochondrial-anchored protein ligase,MU
EIAAB45118 E3 ubiquitin-protein ligase UBR5,E3 ubiquitin-protein ligase, HECT domain-containing 1,EDD,EDD1,hHYD,Homo sapiens,Human,HYD,Hyperplastic discs protein homolog,KIAA0896,Progestin-induced protein,UBR5
EIAAB45120 100 kDa protein,Dd5,E3 ubiquitin-protein ligase UBR5,E3 ubiquitin-protein ligase, HECT domain-containing 1,Edd,Edd1,Hyd,Hyperplastic discs protein homolog,Rat,Rattus norvegicus,Ubr5
EIAAB44006 Homo sapiens,Human,Probable E3 ubiquitin-protein ligase TRIML2,SPRY domain-containing protein 6,SPRYD6,TRIML2,Tripartite motif family-like protein 2
EIAAB25830 E3 ubiquitin-protein ligase MUL1,Gide,Growth inhibition and death E3 ligase,Mitochondrial ubiquitin ligase activator of NFKB 1,Mouse,Mul1,Mus musculus
EIAAB45296 Ac2-121,E3 ubiquitin-protein ligase UHRF1,Liver regeneration-related protein LRRG126,Rat,Rattus norvegicus,Ubiquitin-like PHD and RING finger domain-containing protein 1,Ubiquitin-like-containing PHD
EIAAB43867 ADP-ribosylation factor domain-containing protein 1,Arfd1,E3 ubiquitin-protein ligase TRIM23,GTP-binding protein ARD-1,Mouse,Mus musculus,Trim23,Tripartite motif-containing protein 23


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur