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Pubmed ID :16644739
Publication Date : //

Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana.


Plant genomes contain genetically encoded isoforms of most nucleotide sugar interconversion enzymes. Here we show that Arabidopsis thaliana has five genes encoding functional UDP-D-glucose/UDP-D-galactose 4-epimerase (named UGE1 to UGE5). All A. thaliana UDP-d-glucose 4-epimerase isoforms are dimeric in solution, maximally active in vitro at 30-40 degrees C, and show good activity between pH 7 and pH 9. In vitro, UGE1, -3, and -5 act independently of externally added NAD+, whereas cofactor addition stimulates the activity of UGE2 and is particularly important for UGE4 activity. UGE1 and UGE3 are most efficiently inhibited by UDP. The five isoforms display kcatUDP-Gal values between 23 and 128 s(-1) and KmUDP-Gal values between 0.1 and 0.3 mm. This results in enzymatic efficiencies ranging between 97 and 890 mm(-1) s(-1) for UGE4 = UGE1 < UGE3 < UGE5 < UGE2. The KmUDP-Glc values, derived from the Haldane relationship, were 0.76 mm for UGE1, 0.56 mm for UGE4, and between 0.13 and 0.23 mm for UGE2, -3, and -5. The expression of UGE isoforms is ubiquitous and displays developmental and cell type-dependent variations. UGE1 and -3 expression patterns globally resemble enzymes involved in carbohydrate catabolism, and UGE2, -4, and -5 expression is more related to carbohydrate biosynthesis. UGE1, -2, and -4 are present in the cytoplasm, whereasUGE4 is additionally enriched close to Golgi stacks. All UGE genes tested complement the UGE4rhd1 phenotype, confer increased galactose tolerance in planta, and complement the galactose metabolization deficiency in the Saccharomyces cerevisiae gal10 mutant. We suggest that plant UGE isoforms function in different metabolic situations and that enzymatic properties, gene expression pattern, and subcellular localization contribute to the differentiation of isoform function.

Authors : Barber Christine , Rösti Johannes , Rawat Arun , Findlay Kim , Roberts Keith , Seifert Georg J ,

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26-862 GALE is an UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N- 0.05 mg
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AS11 1783 Antibody: Acd1 | accelerated cell death 1 , Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 477 Antibody: S14 | 40S ribosomal protein S14-1, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 474 Antibody: AtMTP4 | metal tolerance protein B, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 515 Antibody: KC1 | potassium channel KAT3 , Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS09 475 Antibody: Ligand-gated ion channel 1.3, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Raph 100
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
AS10 651 Antibody: Enolase, Immunogen: recombinant Arabidopsis thaliana enolase At2g36530, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 937 Antibody: CPLD11 , Immunogen: overexpressed fragment of recombinant Arabidopsis thaliana CPLD11 NP_566650, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 671 Antibody: AGO5 | argonaute 5, Immunogen: peptide of Arabidopsis thaliana AGO5 Q9SJK3, AT2G27880, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 672 Antibody: AGO6 | argonaute 6, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 At2g32940, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 459 Antibody: ClpB-P | ClpB3, Immunogen: KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS06 116 Antibody: PGL35 | plastoglobulin 35, Immunogen: recombinant Arabidopsis thaliana PGL35 protein O81439 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Gossypium hirsutum cv. Del 200
AS11 1753 Antibody: HDT3 | histone deacetylase, Immunogen: recombinant part of Arabidopsis thaliana HDT3 Q9LZR5, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1628 Antibody: HSP18.5 | class IV heat shock protein, Immunogen: recombinant Arabidopsis thaliana Hsp18.5 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 721 Antibody: PHOT2 | phototropin-2, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS10 720 Antibody: PHOT1 | phototropin-1, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS08 346 Antibody: HSP90-1 | heat shock protein 90-1, Immunogen: full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Zea m 100
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AS10 711 Antibody: DEG15 | endopeptidase, peroxisomal marker , Immunogen: synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
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