Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :16913867
Publication Date : //

The role of NADPH oxidase and MAP kinase phosphatase in UV-B-dependent gene expression in Arabidopsis.

Plant responses to supplementary UV-B irradiation have been reported to include formation of reactive oxygen species (ROS), hydrogen peroxide, in particular, and regulation by mitogen-activated protein kinase (MAPK) cascades which in turn are fine-tuned by MAPK phosphatases (MKPs). Here we present direct genetic evidence for the involvement of plasma membrane NADPH oxidase, a source of superoxide and hydrogen peroxide in the apoplasts, in UV-B signalling in Arabidopsis thaliana, by analysis of gene expression of the UV-B molecular markers in NADPH oxidase (atrbohD, F and DF) and MAP kinase phosphatase 1 (MKP1) knockout mutants (mkp1). Whereas the NADPH oxidase mutants were affected in UV-B-dependent CHS, PYROA and MEB5.2 gene expression, the mkp1 mutant was affected in the general expression pattern of the pathogenesis-related (PR) and PDF1.2 genes. The results indicate involvement of MKP1 in repressive action on gene expression of more general stress response pathways, similar to those activated by pathogen attack, while NADPH oxidase is involved in quantitative (rather than absolute) regulation of more UV-B-specific genes. The expressions of the molecular markers in the knockout mutant mkp1 and in its complemented lines (lines 6 and 10) were similar, as opposed to the responses of the corresponding wild-type Wassilewskija-4 (Ws-4). Lines 6 and 10 showed much higher MKP1 mRNA than Ws-4 but did not complement the mutant. This suggests a complex dependency of the MAPK phosporylation level of the PR and PDF1.2 genes. Both NADPH oxidase mutants and the mkp1 mutant phenotypically responded to UV-B by growth retardation.

Authors : Kalbina Irina , Strid Ake ,

Related products :

Catalog number Product name Quantity
EIAAB27575 Kidney oxidase-1,Kidney superoxide-producing NADPH oxidase,KOX-1,Mouse,Mus musculus,NADPH oxidase 4,Nox4,Renal NAD(P)H-oxidase,Renox,Superoxide-generating NADPH oxidase 4
EIAAB27574 Homo sapiens,Human,Kidney oxidase-1,Kidney superoxide-producing NADPH oxidase,KOX-1,NADPH oxidase 4,NOX4,Renal NAD(P)H-oxidase,RENOX
EIAAB27573 Kidney oxidase-1,Kidney superoxide-producing NADPH oxidase,Kox,KOX-1,NADPH oxidase 4,Nox4,Rat,Rattus norvegicus
EIAAB12031 Dual oxidase 2,DUOX2,Homo sapiens,Human,Large NOX 2,LNOX2,Long NOX 2,NADH_NADPH thyroid oxidase p138-tox,NADPH oxidase_peroxidase DUOX2,NADPH thyroid oxidase 2,p138 thyroid oxidase,THOX2,Thyroid oxida
EIAAB06559 CDI1,CDK2-associated dual-specificity phosphatase,CDKN3,CIP2,Cyclin-dependent kinase inhibitor 3,Cyclin-dependent kinase interactor 1,Cyclin-dependent kinase-interacting protein 2,Homo sapiens,Human,K
EIAAB26522 67 kDa neutrophil oxidase factor,Homo sapiens,Human,NADPH oxidase activator 2,NCF2,NCF-2,Neutrophil cytosol factor 2,Neutrophil NADPH oxidase factor 2,NOXA2,P67PHOX,p67-phox
26-725 RDH12 is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit ster 0.05 mg
EIAAB26524 67 kDa neutrophil oxidase factor,Mouse,Mus musculus,NADPH oxidase activator 2,Ncf2,NCF-2,Neutrophil cytosol factor 2,Neutrophil NADPH oxidase factor 2,Noxa2,P67phox,p67-phox
EIAAB26662 Homo sapiens,Human,NADPH-dependent diflavin oxidoreductase 1,NADPH-dependent FMN and FAD-containing oxidoreductase,NDOR1,Novel reductase 1,NR1
EIAAB32066 Ca(2+)_calmodulin-dependent protein kinase phosphatase,CaM-kinase phosphatase,CaMKPase,Partner of PIX 2,Popx2,Ppm1f,Protein phosphatase 1F,Rat,Rattus norvegicus
31-010 CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fib 0.05 mg
EIAAB06558 CDK2-associated dual-specificity phosphatase,CDKN3,Cyclin-dependent kinase inhibitor 3,KAP,Kinase-associated phosphatase,Pig,Sus scrofa
EIAAB32067 Ca(2+)_calmodulin-dependent protein kinase phosphatase,CaM-kinase phosphatase,CaMKPase,Mouse,Mus musculus,Ppm1f,Protein phosphatase 1F
EIAAB06561 CDK2-associated dual-specificity phosphatase,Cdkn3,Cyclin-dependent kinase inhibitor 3,Kap,Kinase-associated phosphatase,Rat,Rattus norvegicus
EIAAB06560 CDK2-associated dual-specificity phosphatase,Cdkn3,Cyclin-dependent kinase inhibitor 3,Kap,Kinase-associated phosphatase,Mouse,Mus musculus
EIAAB32068 Ca(2+)_calmodulin-dependent protein kinase phosphatase,CaM-kinase phosphatase,CaMKPase,hFem-2,Homo sapiens,Human,KIAA0015,Partner of PIX 2,POPX2,PPM1F,Protein fem-2 homolog,Protein phosphatase 1F
NOXA1 NOX4 Gene NADPH oxidase 4
NOX4 NOX1 Gene NADPH oxidase 1
NOX5 NOX3 Gene NADPH oxidase 3
EIAAB11177 CR,Dehydrogenase_reductase SDR family member 4,DHRS4,NADPH-dependent carbonyl reductase_NADP-retinol dehydrogenase,NADPH-dependent retinol dehydrogenase_reductase,NDRD,Peroxisomal short-chain alcohol
EIAAB11176 CR,Dehydrogenase_reductase SDR family member 4,Dhrs4,NADPH-dependent carbonyl reductase_NADP-retinol dehydrogenase,NADPH-dependent retinol dehydrogenase_reductase,NDRD,Peroxisomal short-chain alcohol
EIAAB11175 CR,Dehydrogenase_reductase SDR family member 4,DHRS4,NADPH-dependent carbonyl reductase_NADP-retinol dehydrogenase,NADPH-dependent retinol dehydrogenase_reductase,NDRD,Oryctolagus cuniculus,Peroxisoma
EIAAB11178 CR,D14Ucla2,Dehydrogenase_reductase SDR family member 4,Dhrs4,mouNRDR,Mouse,Mus musculus,NADPH-dependent carbonyl reductase_NADP-retinol dehydrogenase,NADPH-dependent retinol dehydrogenase_reductase,N
EIAAB11173 CR,Dehydrogenase_reductase SDR family member 4,DHRS4,Homo sapiens,Human,humNRDR,NADPH-dependent carbonyl reductase_NADP-retinol dehydrogenase,NADPH-dependent retinol dehydrogenase_reductase,NRDR,Perox
EIAAB27582 Homo sapiens,Human,NADPH oxidase organizer 1,NADPH oxidase regulatory protein,Nox organizer 1,NOXO1,Nox-organizing protein 1,P41NOX,SH3 and PX domain-containing protein 5,SH3PXD5


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur