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Pubmed ID :16964259
Publication Date : //

Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.


The Mediator head module stimulates basal RNA polymerase II (Pol II) transcription and enables transcriptional regulation. Here we show that the head subunits Med8, Med18 and Med20 form a subcomplex (Med8/18/20) with two submodules. The highly conserved N-terminal domain of Med8 forms one submodule that binds the TATA box-binding protein (TBP) in vitro and is essential in vivo. The second submodule consists of the C-terminal region of Med8 (Med8C), Med18 and Med20. X-ray analysis of this submodule reveals that Med18 and Med20 form related beta-barrel folds. A conserved putative protein-interaction face on the Med8C/18/20 submodule includes sites altered by srb mutations, which counteract defects resulting from Pol II truncation. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.

Authors : Larivière Laurent , Geiger Sebastian , Hoeppner Sabine , Röther Susanne , Strässer Katja , Cramer Patrick ,

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Catalog number Product name Quantity
MED20 MED18 Gene mediator complex subunit 18
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MED8 MED6 Gene mediator complex subunit 6
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CSB-EL013651MO Mouse Mediator of RNA polymerase II transcription subunit 18(MED18) ELISA kit 96T
CSB-EL013653RA Rat mediator complex subunit 20 (MED20) ELISA kit, Species Rat, Sample Type serum, plasma 96T
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CSB-EL013651MO Mouse Mediator of RNA polymerase II transcription subunit 18(MED18) ELISA kit SpeciesMouse 96T
CSB-EL013651HU Human Mediator of RNA polymerase II transcription subunit 18(MED18) ELISA kit SpeciesHuman 96T
CSB-EL013668HU Human Mediator of RNA polymerase II transcription subunit 8(MED8) ELISA kit 96T
CSB-EL013668MO Mouse Mediator of RNA polymerase II transcription subunit 8(MED8) ELISA kit 96T


 

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