Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :17372656
Publication Date : //

An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells.


Current methods for phosphoproteome analysis have several limitations. First, most methods for phosphopeptide enrichment lack the specificity to truly purify phosphopeptides. Second, fragmentation spectra of phosphopeptides, in particular those of phosphoserine and phosphothreonine containing peptides, are often dominated by the loss of the phosphate group(s) and therefore lack the information required to identify the peptide sequence and the site of phosphorylation, and third, sequence database search engines and statistical models for data validation are not optimized for the specific fragmentation properties of phosphorylated peptides. Consequently, phosphoproteomic data are characterized by large and unknown rates of false positive and false negative phosphorylation sites. Here we present an integrated chemical, mass spectrometric and computational strategy to improve the efficiency, specificity and confidence in the identification of phosphopeptides and their site(s) of phosphorylation. Phosphopeptides were isolated with high specificity through a simple derivatization procedure based on phosphoramidate chemistry. Identification of phosphopeptides, their site(s) of phosphorylation and the corresponding phosphoproteins was achieved by the optimization of the mass spectrometric data acquisition procedure, the computational tools for database searching and the data post processing. The strategy was applied to the mapping of phosphorylation sites of a purified transcription factor, dFOXO and for the global analysis of protein phosphorylation of Drosophila melanogaster Kc167 cells.

Authors : Bodenmiller Bernd , Mueller Lukas N , Pedrioli Patrick G A , Pflieger Delphine , Jünger Martin A , Eng Jimmy K , Aebersold Ruedi , Tao W Andy ,

Related products :

Catalog number Product name Quantity
orb81135 Drosophila melanogaster GAGA-POZ protein GAGA-POZ Drosophila Melanogaster Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 130 amino acids having a molecular m 10
AS01 012 Antibody: DS5a | Drosophila 26S proteasome subunit Rpn10, Host: rabbit, polyclonal, Comfirmed reactivity: Arabidopsis thaliana, Drosophila melanogaster 100
pro-435 Recombinant Drosophila melanogaster GAGA-POZ 10
RP-881 Recombinant Drosophila melanogaster GAGA_POZ 10 ug
RP-881 Recombinant Drosophila melanogaster GAGA-POZ 10 ug
PRO-435 Recombinant Drosophila melanogaster GAGA_POZ 50µg
PRO-435 Recombinant Drosophila melanogaster GAGA_POZ 1mg
pro-435 Recombinant Drosophila melanogaster GAGA-POZ 50
PRO-435 Recombinant Drosophila melanogaster GAGA_POZ 10µg
pro-435 Recombinant Drosophila melanogaster GAGA-POZ 1mg
2002-50 GenoExplorer™ microRNA kit - Drosophila melanogaster 20 23 reactions x3
1702 GenoExplorer™ microRNA kit - Drosophila melanogaster 4 7 reactions x10
228-10513-3 Recombinant Drosophila melanogaster GAGA-POZ 1 mg
228-10513-1 Recombinant Drosophila melanogaster GAGA-POZ 10
OETPRO435 GAGA-POZ Drosophila Melanogaster Recombinant 50
7-05250 Recombinant Drosophila melanogaster GAGA-POZ 1mg
1132C GenoExplorer™ microRNA kit - Drosophila melanogaster 20 20 reactions
ACLPRO435B Drosophila melanogaster GAGA_POZ, recombinant 50 µg.
OETPRO435 GAGA-POZ Drosophila Melanogaster Recombinant 10
228-10513-2 Recombinant Drosophila melanogaster GAGA-POZ 50
ACLPRO435 Drosophila melanogaster GAGA_POZ, recombinant 10 µg.
1131C GenoExplorer™ microRNA kit - Drosophila melanogaster 4 4 reactions
7-05248 Recombinant Drosophila melanogaster GAGA-POZ 10
7-05249 Recombinant Drosophila melanogaster GAGA-POZ 50
RPRO-435C Recombinant Drosophila melanogaster GAGA_POZ 1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur