Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :17452327
Publication Date : //

Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.


Mammalian Dicer interacts with double-stranded RNA-binding protein TRBP or PACT to mediate RNA interference and micro-RNA processing. TRBP and PACT are structurally related but exert opposite regulatory activities on PKR. It is not understood whether TRBP and PACT are simultaneously required for Dicer. Here we show that TRBP directly interacts with PACT in vitro and in mammalian cells. TRBP and PACT form a triple complex with Dicer and facilitate the production of small interfering RNA (siRNA) by Dicer. Knockdown of both TRBP and PACT in cultured cells leads to significant inhibition of gene silencing mediated by short hairpin RNA but not by siRNA, suggesting that TRBP and PACT function primarily at the step of siRNA production. Taken together, these findings indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Our work significantly alters the current model for the assembly and function of the Dicer-containing complex that generates siRNA and micro-RNA in human.

Authors : Kok Kin Hang , Ng Ming-Him James , Ching Yick-Pang , Jin Dong-Yan ,

Related products :

Catalog number Product name Quantity
A4138 TRBP Primary Antibody, TRBP, Species: Human Recombinant Protein Source: Rabbit Polyclonal 100ug
EIAAB09783 C3orf8,Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3,CTD small phosphatase-like protein,CTDSPL,CTDSP-like,Homo sapiens,Human,hYA22,NIF1,NIFL,NIF-like protein,NLI-interact
LF-MA0209 anti-TRBP (46D1), Mouse monoclonal to TRBP, Isotype IgG1, Host Mouse 100 ul
E0156h ELISA DICER,DICER1,Endoribonuclease Dicer,Helicase MOI,Helicase with RNase motif,HERNA,Homo sapiens,Human,KIAA0928 96T
E0156h ELISA kit DICER,DICER1,Endoribonuclease Dicer,Helicase MOI,Helicase with RNase motif,HERNA,Homo sapiens,Human,KIAA0928 96T
U0156h CLIA DICER,DICER1,Endoribonuclease Dicer,Helicase MOI,Helicase with RNase motif,HERNA,Homo sapiens,Human,KIAA0928 96T
E0156b ELISA Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
U0156b CLIA Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
E0156b ELISA kit Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
3697BP-50 Dicer Blocking Peptide target: Dicer 50 μg
3697BP-50 Dicer Blocking Peptide Dicer 50 μg
E0156m ELISA kit Dicer,Dicer1,Double-strand-specific ribonuclease mDCR-1,Endoribonuclease Dicer,Mdcr,Mouse,Mus musculus 96T
E0156m ELISA Dicer,Dicer1,Double-strand-specific ribonuclease mDCR-1,Endoribonuclease Dicer,Mdcr,Mouse,Mus musculus 96T
U0156m CLIA Dicer,Dicer1,Double-strand-specific ribonuclease mDCR-1,Endoribonuclease Dicer,Mdcr,Mouse,Mus musculus 96T
MD80003 MagCustom project Production Phase, third phase (production phase) of client bead development in production scale incl. SOP, QA, warehousing etc. 1 item
E0156c ELISA Chicken,DICER,DICER1,Endoribonuclease Dicer,Gallus gallus 96T
U0156c CLIA Chicken,DICER,DICER1,Endoribonuclease Dicer,Gallus gallus 96T
E0156c ELISA kit Chicken,DICER,DICER1,Endoribonuclease Dicer,Gallus gallus 96T
SMC-416D Dicer Antibody Monoclonal, Clone number: S167-7 Host: Mouse, Isotype: IgG1 Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer accession number Q8R418. 100ug
25-077 HCFC2 is one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP 0.05 mg
27-645 HCFC2 is one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP 0.05 mg
18-272-195878 Dicer - Rabbit polyclonal to Dicer; EC 3.1.26.-; Helicase with RNase motif; Helicase-MOI Polyclonal 0.1 mg
EIAAB35863 cAMP-GEFII,cAMP-regulated guanine nucleotide exchange factor II,CGEF2,EPAC 2,EPAC2,Exchange factor directly activated by cAMP 2,Exchange protein directly activated by cAMP 2,Homo sapiens,Human,Rap gua
TM 985 E.T. MEDIUM (for production of Clostridia for enterotoxin production) 500 gm
EIAAB35859 cAMP-GEFI,cAMP-regulated guanine nucleotide exchange factor I,CGEF1,EPAC,EPAC 1,EPAC1,Exchange factor directly activated by cAMP 1,Exchange protein directly activated by cAMP 1,Homo sapiens,Human,Rap


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur