Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :18407956
Publication Date : //

A multidimensional chromatography technology for in-depth phosphoproteome analysis.


Protein phosphorylation is a post-translational modification widely used to regulate cellular responses. Recent studies showed that global phosphorylation analysis could be used to study signaling pathways and to identify targets of protein kinases in cells. A key objective of global phosphorylation analysis is to obtain an in-depth mapping of low abundance protein phosphorylation in cells; this necessitates the use of suitable separation techniques because of the complexity of the phosphoproteome. Here we developed a multidimensional chromatography technology, combining IMAC, hydrophilic interaction chromatography, and reverse phase LC, for phosphopeptide purification and fractionation. Its application to the yeast Saccharomyces cerevisiae after DNA damage led to the identification of 8764 unique phosphopeptides from 2278 phosphoproteins using tandem MS. Analysis of two low abundance proteins, Rad9 and Mrc1, revealed that approximately 50% of their phosphorylation was identified via this global phosphorylation analysis. Thus, this technology is suited for in-depth phosphoproteome studies.

Authors : Albuquerque Claudio P , Smolka Marcus B , Payne Samuel H , Bafna Vineet , Eng Jimmy , Zhou Huilin ,

Related products :

Catalog number Product name Quantity
S-0040.1 Multiplexed Analysis of RNA using Luminex Multi Analyte Profiling Technology (xMAP). 5x
S-0040.2 Multiplexed Analysis of Proteins using Multi Analyte Profiling Technology (xMAP) 5x
A30 Chromatography Software, Extension modules, EA SW module for Elemental Analysis
S-0034.1 Multidimensional Protein Identification (MudPIT).
TotalLabQuant Base package*: 1D image analysis plus colony counting and array analysis
AS-MRA-P5 MARIA™ ANALYSIS SERVICE 5-plex analysis Environmental Testing Services 1 sample
XGC-BiRDS-MeDIP-Seq Bioinformatics analysis of Methylome data for Methylation analysis Sample
AS-MRA-P8 MARIA™ ANALYSIS SERVICE 8-plex analysis Environmental Testing Services 1 sample
XGC-BiRDS-WGBS Bioinformatics analysis of Whole Genome Bisulphite Sequencing data for Methylation analysis Sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis 1
Phoretix1D Base package*: 1D image analysis with band pattern matching, plus colony counting and array analysis
Phoretix 1D Base package* 1D image analysis with band pattern matching, plus colony counting and array analysis 1
Phoretix 1D  Base package* 1D image analysis with band pattern matching, plus colony counting and array analysis
AS-MRA-P8 8-plex analysis MARIA™ ANALYSIS SERVICE 1 sample
AS-MRA-P5 8-plex analysis MARIA™ ANALYSIS SERVICE 1 sample
33-588-04 COTTLE with depth marking 4 mm 18 cm, 7" x10
33-588-10 COTTLE with depth marking 10 mm 18 cm, 7" x 50
33-588-06 COTTLE with depth marking 6 mm 18 cm, 7" x 50
33-588-08 COTTLE with depth marking 8 mm 18 cm, 7" x 50
33-588-08 COTTLE with depth marking 8 mm 18 cm, 7" x10
33-588-04 COTTLE with depth marking 4 mm 18 cm, 7" x 50
33-588-12 COTTLE with depth marking 12 mm 18 cm, 7" x10
33-588-12 COTTLE with depth marking 12 mm 18 cm, 7" x 50
33-588-10 COTTLE with depth marking 10 mm 18 cm, 7" x10


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur