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Pubmed ID :18479511
Publication Date : //

Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling.


The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. It is tightly integrated with transcription, particularly its termination, as well as other RNA processing events, i.e. capping and splicing. The poly(A) tail protects the mRNA from unregulated degradation, and it is required for nuclear export and translation initiation. In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis-elements and produce the poly(A) tail. Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA polyadenylation protein machinery in Arabidopsis.

Authors : Hunt Arthur G , Xu Ruqiang , Addepalli Balasubrahmanyam , Rao Suryadevara , Forbes Kevin P , Meeks Lisa R , Xing Denghui , Mo Min , Zhao Hongwei , Bandyopadhyay Amrita , Dampanaboina Lavanya , Marion Amanda , Von Lanken Carol , Li Qingshun Quinn ,

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