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Pubmed ID :18642946
Publication Date : //

Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana.


Among the signal transduction pathways in higher eukaryotes, the two-component system (TCS) is unique to plants. In the model plant Arabidopsis thaliana, it consists of more than 30 proteins, including eight receptors, five phosphotransmitters and 23 response regulators. One of its important functions is to perceive and transduce the signal of the plant hormone cytokinin. The basic signal flow within the TCS is well-understood, but it is unclear how this pathway is integrated with the remainder of the proteome. Thus, knowledge about the interactions of TCS proteins should contribute to the understanding of their mode of action. Therefore, we conducted medium-scale yeast two-hybrid screens focusing on those members of the TCS, which are thought to be involved in cytokinin signaling. In total, more than 6.3 x 10 (7) transformants were screened resulting in the identification of 160 different interactions, of which 136 were novel. Most of the interacting proteins belong to the functional categories of signal transduction and protein metabolism. TCS proteins and their interactors localized to the same subcellular compartment in many cases, a prerequisite to being of biological relevance. The resulting interaction network map revealed large differences in the connectivity. Cytokinin receptors (AHK2, CRE1/AHK4) showed the highest numbers of different interaction partners. This study is the first systematic protein-protein interaction experiment for a plant signal system and provides numerous starting points for further analysis of the molecular mechanisms used to convert the signal carried by the TCS into biological processes.

Authors : Dortay Hakan , Gruhn Nijuscha , Pfeifer Andreas , Schwerdtner Mareike , Schmülling Thomas , Heyl Alexander ,

Related products :

Catalog number Product name Quantity
AS07 258 Antibody: LOX-C | chloroplastic lipoxygenase, Immunogen: recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 476 Antibody: AtArf2 | ADP ribosylation factor 2, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 477 Antibody: S14 | 40S ribosomal protein S14-1, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1783 Antibody: Acd1 | accelerated cell death 1 , Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 474 Antibody: AtMTP4 | metal tolerance protein B, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 515 Antibody: KC1 | potassium channel KAT3 , Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS09 475 Antibody: Ligand-gated ion channel 1.3, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Raph 100
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
AS10 651 Antibody: Enolase, Immunogen: recombinant Arabidopsis thaliana enolase At2g36530, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 937 Antibody: CPLD11 , Immunogen: overexpressed fragment of recombinant Arabidopsis thaliana CPLD11 NP_566650, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 671 Antibody: AGO5 | argonaute 5, Immunogen: peptide of Arabidopsis thaliana AGO5 Q9SJK3, AT2G27880, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 672 Antibody: AGO6 | argonaute 6, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 At2g32940, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 459 Antibody: ClpB-P | ClpB3, Immunogen: KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS11 1753 Antibody: HDT3 | histone deacetylase, Immunogen: recombinant part of Arabidopsis thaliana HDT3 Q9LZR5, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS06 116 Antibody: PGL35 | plastoglobulin 35, Immunogen: recombinant Arabidopsis thaliana PGL35 protein O81439 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Gossypium hirsutum cv. Del 200
AS11 1628 Antibody: HSP18.5 | class IV heat shock protein, Immunogen: recombinant Arabidopsis thaliana Hsp18.5 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 721 Antibody: PHOT2 | phototropin-2, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS10 720 Antibody: PHOT1 | phototropin-1, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 614 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 100
AS09 615 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 100
AS05 059 Antibody: PsbR | 10 kDa protein of PSII, Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsbR P27202, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, 100
AS09 527 Antibody: AGO1 | argonaute 1, Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana 100
AS10 711 Antibody: DEG15 | endopeptidase, peroxisomal marker , Immunogen: synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS08 346 Antibody: HSP90-1 | heat shock protein 90-1, Immunogen: full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Zea m 100
AS11 1629 Antibody: HSP90-2 | heat shock protein 90-2, Immunogen: full length recombinant Hsp90-2 from Arabidopsis thaliana, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100


 

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