Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :21220307
Publication Date : //

Phosphorylation of glutamyl-prolyl tRNA synthetase by cyclin-dependent kinase 5 dictates transcript-selective translational control.

Cyclin-dependent kinase 5 (Cdk5) is an atypical but essential member of the Cdk kinase family, and its dysregulation or deletion has been implicated in inflammation-related disorders by an undefined mechanism. Here we show that Cdk5 is an indispensable activator of the GAIT (IFN-γ-activated inhibitor of translation) pathway, which suppresses expression of a posttranscriptional regulon of proinflammatory genes in myeloid cells. Through induction of its regulatory protein, Cdk5R1 (p35), IFN-γ activates Cdk5 to phosphorylate Ser(886) in the linker domain of glutamyl-prolyl tRNA synthetase (EPRS), the initial event in assembly of the GAIT complex. Cdk5/p35 also induces, albeit indirectly via a distinct kinase, phosphorylation of Ser(999), the second essential event in GAIT pathway activation. Diphosphorylated EPRS is released from its residence in the tRNA multisynthetase complex for immediate binding to NS1-associated protein and subsequent binding to ribosomal protein L13a and GAPDH. The mature heterotetrameric GAIT complex binds the 3' UTR GAIT element of VEGF-A and other target mRNAs and suppresses their translation in myeloid cells. Inhibition of Cdk5/p35 inhibits both EPRS phosphorylation events, prevents EPRS release from the tRNA multisynthetase complex, and blocks translational suppression of GAIT element-bearing mRNAs, resulting in increased expression of inflammatory proteins. Our study reveals a unique role of Cdk5/p35 in activation of the major noncanonical function of EPRS, namely translational control of macrophage inflammatory gene expression.

Authors : Arif Abul , Jia Jie , Moodt Robyn A , DiCorleto Paul E , Fox Paul L ,

Related products :

Catalog number Product name Quantity
EPS15L1 EPRS Gene glutamyl-prolyl-tRNA synthetase
E9959h Human Glutamyl Prolyl tRNA Synthetase ELISA Kit 96T
bs-13393R Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody 100ul
bs-13393R-Cy3 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, Cy3 Conjugated 100ul
bs-13393R-Cy5.5 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, Cy5.5 Conjugated 100ul
bs-13393R-Cy7 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, Cy7 Conjugated 100ul
bs-13393R-Cy5 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, Cy5 Conjugated 100ul
bs-13393R-A555 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, ALEXA FLUOR 555 Conjugated 100ul
bs-13393R-A488 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, ALEXA FLUOR 488 Conjugated 100ul
bs-13393R-A647 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, ALEXA FLUOR 647 Conjugated 100ul
bs-13393R-A350 Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, ALEXA FLUOR 350 Conjugated 100ul
EIAAB32694 Homo sapiens,Human,NCRNA00117,PrdX deacylase domain-containing protein 1,PRDXDD1P,Prolyl-tRNA synthetase associated domain-containing protein 1 pseudogene,PRORSD1P,Putative prolyl-tRNA synthetase asso
bs-13393R-FITC Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, FITC Conjugated 100ul
bs-13393R-Biotin Rabbit Anti-Glutamyl Prolyl tRNA synthetase-ProRS Polyclonal Antibody, Biotin Conjugated 100ul
CSB-EL007747MO Mouse glutamyl-prolyl-tRNA synthetase (EPRS) ELISA kit, Species Mouse, Sample Type serum, plasma 96T
CSB-EL007747HU Human glutamyl-prolyl-tRNA synthetase (EPRS) ELISA kit, Species Human, Sample Type serum, plasma 96T
EIAAB06559 CDI1,CDK2-associated dual-specificity phosphatase,CDKN3,CIP2,Cyclin-dependent kinase inhibitor 3,Cyclin-dependent kinase interactor 1,Cyclin-dependent kinase-interacting protein 2,Homo sapiens,Human,K
EIAAB40723 Ears2,GluRS,Glutamate--tRNA ligase,Kiaa1970,Mouse,Mus musculus,Probable glutamyl-tRNA synthetase, mitochondrial
YSGKAMCC107E Cyclin Dependent Kinase 2 (cdk2), Cyclin Dependent Kinase 5 (cdk5), NO X w_Cyclin Dependent Kinase (cdk)1, ~33kD, Clone 8A12, Mab anti_Human, Mouse; WB 100 µg.
EIAAB40722 EARS2,GluRS,Glutamate--tRNA ligase,Homo sapiens,Human,KIAA1970,Probable glutamyl-tRNA synthetase, mitochondrial
orb60521 BS-181 hydrochloride BS-181 is a selective cyclin-dependent kinase inhibitor with an IC50 of 21 nM for the inhibition of CDK-activating kinase. For research use only. 10 mg
EIAAB40832 Pars2,Probable prolyl-tRNA synthetase, mitochondrial,Proline--tRNA ligase,ProRS,Rat,Rattus norvegicus
EIAAB40833 Mouse,Mus musculus,Pars2,Probable prolyl-tRNA synthetase, mitochondrial,Proline--tRNA ligase,ProRS
EIAAB40834 Homo sapiens,Human,PARS2,Probable prolyl-tRNA synthetase, mitochondrial,Proline--tRNA ligase,ProRS
EIAAB40724 Chicken,EARS2,Gallus gallus,GluRS,Glutamate--tRNA ligase,Probable glutamyl-tRNA synthetase, mitochondrial,RCJMB04_20f12


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur