Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :2576095
Publication Date : //

Analysis of genes coding for the sialic acid-binding adhesin and two other minor fimbrial subunits of the S-fimbrial adhesin determinant of Escherichia coli.


The S fimbrial adhesin (Sfa) enables Escherichia coli to attach to sialic acid-containing receptor molecules of eukaryotic cells. As previously reported, the genetic determinant coding for the Sfa of an E. coli O6 strain was cloned, the gene coding for the major fimbrial subunit was identified and sequenced and the S specific adhesin was detected. Here we present evidence that in addition to the major subunit protein SfaA three other minor subunit proteins, SfaG (17 kD), SfaS (14 kD) and SfaH (31 kD) can be isolated from the S-specific fimbrial adhesin complex. The genes coding for these minor subunits were identified, mutagenized separately and sequenced. Using haemagglutination tests, electron-microscopy and quantitative ELISA assays with monoclonal anti-SfaA and anti-SfaS antibodies the functions of the minor subunits were determined. It was determined that SfaS is identical to the S-specific adhesin, which also plays a role in determination of the degree of fimbriation of the cell. The minor subunit SfaH also had some influence on the level of fimbriation of the cell, while SfaG is necessary for full expression of S-specific binding. It was further shown that the amino-terminal protein sequence of the isolated SfaS protein was identical to the protein sequence calculated from the DNA sequence of the sfaS gene locus.

Authors : Schmoll T , Hoschützky H , Morschhäuser J , Lottspeich F , Jann K , Hacker J ,

Related products :

Catalog number Product name Quantity
CEVETPA0238 Fimbrial adhesins, E. coli F41, Mab anti_ 1 mg.
CEVETPA0236 Fimbrial adhesins, E. coli K88b, Mab anti_ 1 mg.
CEVETPA0237 Fimbrial adhesins, E. coli 987P, Mab anti_ 1 mg.
SSI51172 E. Coli, F4 (K88) fimbrial antisera for determination of pili 5 ml.
SSI51173 E. Coli, F5 (K99) fimbrial antisera for determination of pili 5 ml.
CEVETPA0235 Fimbrial adhesins, E. coli K88a, Mab anti_ 1 mg.
orb71328 Ac-Adhesin (1025-1044) peptide This is Ac-Adhesin (1025-1044) peptide. For research use only. 1 mg
EIAAB38451 MIS,Mouse,mSiglec-E,Mus musculus,Myeloid inhibitory siglec,Sialic acid-binding Ig-like lectin 12,Sialic acid-binding Ig-like lectin 5,Sialic acid-binding Ig-like lectin E,Sialic acid-binding Ig-like l
P91245Mu01 Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12) Organism: Mus musculus (Mouse) Source: Escherichia coli 50ug
P91245Mu01 Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12) Organism: Mus musculus (Mouse) Source: Escherichia coli 10ug
P91245Mu01 Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12) Organism: Mus musculus (Mouse) Source: Escherichia coli 100ug
P91789Hu01 Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90961Hu01 Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93922Hu01 Sialic Acid Binding Ig Like Lectin 9 (SIGLEC9) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91144Hu01 Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90961Hu02 Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90961Hu03 Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93921Hu01 Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93922Hu01 Sialic Acid Binding Ig Like Lectin 9 (SIGLEC9) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P86344Hu01 Sialic Acid Binding Ig Like Lectin 14 (SIGLEC14) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P91789Hu02 Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P86344Hu01 Sialic Acid Binding Ig Like Lectin 14 (SIGLEC14) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91789Hu03 Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91245Hu01 Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90966Hu01 Sialic Acid Binding Ig Like Lectin 1 (SIGLEC1) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur