Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :25963241
Publication Date : //

Optogenetic control of molecular motors and organelle distributions in cells.


Intracellular transport and distribution of organelles play important roles in diverse cellular functions, including cell polarization, intracellular signaling, cell survival, and apoptosis. Here, we report an optogenetic strategy to control the transport and distribution of organelles by light. This is achieved by optically recruiting molecular motors onto organelles through the heterodimerization of Arabidopsis thaliana cryptochrome 2 (CRY2) and its interacting partner CIB1. CRY2 and CIB1 dimerize within subseconds upon exposure to blue light, which requires no exogenous ligands and low intensity of light. We demonstrate that mitochondria, peroxisomes, and lysosomes can be driven toward the cell periphery upon light-induced recruitment of kinesin, or toward the cell nucleus upon recruitment of dynein. Light-induced motor recruitment and organelle movements are repeatable, reversible, and can be achieved at subcellular regions. This light-controlled organelle redistribution provides a new strategy for studying the causal roles of organelle transport and distribution in cellular functions in living cells.

Authors : Duan Liting , Che Daphne , Zhang Kai , Ong Qunxiang , Guo Shunling , Cui Bianxiao ,

Related products :

Catalog number Product name Quantity
27-983 KIF23 is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This pro 0.05 mg
27-984 KIF23 is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This pro 0.1 mg
27-979 KIF22 a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The 0.1 mg
25-172 The protein encoded by the KIF25 gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move 0.05 mg
27-980 The protein encoded by the KIF25 gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move 0.1 mg
30-861 KLC3 is a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two 0.05 mg
26-779 KLC3 is a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two 0.05 mg
30-860 KLC3 is a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two 0.05 mg
31-270 KIF12 is a member of the kinesin superfamily of microtubule-associated molecular motors that play important roles in intracellular transport and cell division.KIF12 is a member of the kinesin superfam 0.05 mg
32-132 CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It cou 0.1 mL
ERYGEN-AC A panel of 3 percent reagent red blood cells for quality control of anti_human globulin procedures (Coombs control cells) 2 ml
32-128 CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. L 0.1 mL
26-332 Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and 0.05 mg
MC100A-1 Molecular Tools Cold Fusion Cloning Kit with Competent Cells (20 rxns) International Sales Only 20 reactions
MC101A-1 Molecular Tools Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only 50 reactions
MC010A-1 Molecular Tools Cold Fusion Cloning Kit with Competent Cells (10 rxns) International Sales Only 10 reactions
42-608 Competent Cells for Protein Expression BL21(DE3)pLysS Competent Cells for 96_Well Plates, pUC19 DNA (control) included 96 x 20ul/Unit
42-609 Competent Cells for Protein Expression BL21(DE3)pLysS Competent Cells for 96_Well Plate, pUC19 DNA (control) included 4 Sets of 96 x 20ul/Unit
42-662 Competent Cells for Cloning GC10 Competent Cells for Deep Well Tube, pUC19 DNA (control) included 96 x 50ul/Unit
42-663 Competent Cells for Cloning GC10 Competent Cells for 96_Well Plate, pUC19 DNA (control) included 96 x 20ul/Unit
42-601 Competent Cells for Protein Expression BL21 Competent Cells, pUC19 DNA (control) included 5 x 200ul/Unit
orb80180 Hepatitis Surface Antigen adr subtype CHO protein Recombinant HBsAg adr produced in CHO cells, the molecular weight is approximately 23-27kDa. For research use only. 20
orb82810 Mouse MDC protein CCL22_MDC is a CC chemokine that is produced in B cells, macrophages, monocytederived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC c 1 mg
orb81476 Human WNT Inhibitory Factor 1 protein WIF1 produced in Hi-5 cells is a single, glycosylated polypeptide chain containing 369 amino acids (29-379 a.a.) and having a molecular mass of 40.5 kDa, (48kDa o 2
AHP624Z RABBIT CONTROL SERUM (CYTOCHROME P450 ASSAYS), Product Type Serum & Cells, Specificity CONTROL SERUM , Target Species Negative Control, Host N_A, Format Serum, Isotypes , Applications FN, WB, C 0.1 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur