Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :6694738
Publication Date : //

Acceptors for botulinum neurotoxin reside on motor nerve terminals and mediate its internalization.


Botulinum neurotoxin (BoNY) type A, a causative agent of botulism, is a di-chain protein (molecular weight 140,000) from Clostridium botulinum, and the most neurotoxic substance known. Some cases of sudden infant cot deaths have been attributed to such a neuroparalytic condition. BoNT inhibits irreversibly the release of acetylcholine from peripheral nerves in a highly selective manner. Hence, it is potentially an invaluable probe for studying the mechanism of transmitter release. Here we demonstrate specific labelling of murine motor nerve terminals with neurotoxic, 125I-labelled BoNT (type A) by autoradiography. We observed saturable, temperature-sensitive binding of BoNT to sites which reside solely on the nerve terminal membrane; these were distributed on all unmyelinated areas, at an average density of 150-500 per micron2 of membrane. The binding was mediated by the larger subunit of the toxin and was inhibited partially by tetanus toxin, another microbial protein. No specific binding was detectable on any other cell types examined, including noradrenergic terminals. Following binding, internalization of radioactivity was observed; this process was energy-dependent as it could be prevented totally by azide or dinitrophenol (DNP). This direct demonstration of separable steps, including highly selective binding and acceptor-mediated internalization, is reconcilable with the unique potency and the multiphasic inhibitory action of BoNT on transmitter release, as shown electrophysiologically.

Authors : Dolly J O , Black J , Williams R S , Melling J ,

Related products :

Catalog number Product name Quantity
140A Botulinum Neurotoxin Type E Complex, Nicked, from Clostridium botulinum 10 jg.
140B Botulinum Neurotoxin Type E Complex, Nicked, from Clostridium botulinum 100 jg.
LST128A Botulinum Neurotoxin Type A Complex from Clostridium botulinum 10 ug.
133B Botulinum Neurotoxin Type A Toxoid from Clostridium botulinum 50 jg.
LST128C Botulinum Neurotoxin Type A Complex from Clostridium botulinum 100 ug.
136B Botulinum Neurotoxin Type B, nicked, from Clostridium botulinum 1,3,4 100µg
LST138A Botulinum Neurotoxin Type B Complex from Clostridium botulinum 10 ug.
LST9128A QD Botulinum Neurotoxin Type A Complex from Clostridium botulinum 10 ug.
136A Botulinum Neurotoxin Type B. nicked, from Clostridium botulinum 1,3,4 10µg
LST138B Botulinum Neurotoxin Type B Complex from Clostridium botulinum 100 ug.
133A Botulinum Neurotoxin Type A Toxoid from Clostridium botulinum 10 jg.
LST9128B QD Botulinum Neurotoxin Type A Complex from Clostridium botulinum 100 ug.
130A Botulinum Neurotoxin Type A from Clostridium botulinum 10 jg.
139 Botulinum Neurotoxin Type B Toxoid from Clostridium botulinum 10 jg.
LST130A Botulinum Neurotoxin Type A from Clostridium botulinum 10 ug.
LST130B Botulinum Neurotoxin Type A from Clostridium botulinum 100 ug.
LST136A Botulinum Neurotoxin Type B from Clostridium botulinum 10 ug.
136A Botulinum Neurotoxin Type B. from Clostridium botulinum 1,3,4 10
LST9130B QD Botulinum Neurotoxin Type A from Clostridium botulinum 100 ug.
LST9130A QD Botulinum Neurotoxin Type A from Clostridium botulinum 10 ug.
LST136B Botulinum Neurotoxin Type B from Clostridium botulinum 100 ug.
130B Botulinum Neurotoxin Type A from Clostridium botulinum 100 jg.
136A Botulinum Neurotoxin Type B. from Clostridium botulinum 1,3,4 10 µg
25-729 The SLC6A2 gene encodes a norepinephrine (noradrenaline) transporter, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeosta 0.05 mg
139 Botulinum Neurotoxin Type B Toxoid 10


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur