Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8068679
Publication Date : //

Purification of two isoforms of hnRNP-U and characterization of their nucleic acid binding activity.


The scaffold attachment factor A (SAF-A; Romig et al., 1992), a human nuclear protein which specifically binds vertebrate SAR (scaffold attached region) DNA, is identical with hnRNP-U (Kiledjian & Dreyfuss, 1992). In this paper, we report on the purification of two forms of this protein that can be chromatographically separated. We show that the purified proteins represent two isoforms, form 1 and form 2 hnRNP-U, which differ in their primary structure. Both isoforms bind to double- and single-stranded DNA and RNA. In addition, they form higher ordered nucleic acid/protein complexes and specifically bind and aggregate the human SAR element MII at physiological ionic strengths. Electron microscopic analysis shows that the isoforms differ from each other, as form 1 hnRNP-U aggregates into long unbranched filamentous protein/DNA complexes whereas form 2 hnRNP-U aggregates as spheres with an average diameter of 35 nm.

Authors : Fackelmayer F O , Richter A ,

Related products :

Catalog number Product name Quantity
18-003-44201 Poly(rC)-binding protein 1 - Alpha-CP1; hnRNP-E1; Nucleic acid-binding protein SUB2.3 Polyclonal 0.1 mg Protein A
JZ88-T003 Automated Nucleic Acid Purification System: T-Pro FastEZ Purix Viral Nucleic Acid Extraction Kit 1 kit (36 extractions) including all required plastic disposables
JZ88-T003 Automated Nucleic Acid Purification System: T-Pro FastEZ Purix Viral Nucleic Acid Extraction Kit 10 Kits( 1 kit (36 extractions) including all required plastic disposables)
EIAAB30032 Alpha-CP1,Heterogeneous nuclear ribonucleoprotein E1,hnRNP E1,Homo sapiens,Human,Nucleic acid-binding protein SUB2.3,PCBP1,Poly(rC)-binding protein 1
GF-RD-025 GF-1 Viral Nucleic Acid Extraction Kit (Proteinase K included), 25preps Nucleic Acid Purification Kit
GF-RD-100 GF-1 Viral Nucleic Acid Extraction Kit (Proteinase K included), 100preps Nucleic Acid Purification Kit
GF-RD-050 GF-1 Viral Nucleic Acid Extraction Kit (Proteinase K included), 50preps Nucleic Acid Purification Kit
16201 SpinCleanTM Genomic DNA Purification Kit (for blood sample) Nucleic Acid Purification Kits 200prep
16200 SpinCleanTM Genomic DNA Purification Kit (for bacteria sample) Nucleic Acid Purification Kits 200prep
16050 SpinCleanTM Genomic DNA Purification Kit (for bacteria sample) Nucleic Acid Purification Kits 50prep
16202 SpinCleanTM Genomic DNA Purification Kit (for tissue sample) Nucleic Acid Purification Kits 200prep
16052 SpinCleanTM Genomic DNA Purification Kit (for tissue sample) Nucleic Acid Purification Kits 50prep
16051 SpinCleanTM Genomic DNA Purification Kit (for blood sample) Nucleic Acid Purification Kits 50prep
15400 SpinCleanTM RNA Purification Kit (for tissue sample), including DNase Kit Nucleic Acid Purification Kits 200prep
15200 SpinCleanTM RNA Purification Kit (for blood sample), including DNase Kit Nucleic Acid Purification Kits 200prep
15300 SpinCleanTM RNA Purification Kit (for tissue sample), including DNase Kit Nucleic Acid Purification Kits 50prep
15050 SpinCleanTM RNA Purification Kit (for blood sample), including DNase Kit Nucleic Acid Purification Kits 50prep
12200 SpinCleanTM PCR Purification Kit Nucleic Acid Purification Kits 200prep
EP101-02 EasyPureTM PCR Purification Kit, Nucleic Acid Purification 200 rxns
EP101-01 EasyPureTM PCR Purification Kit, Nucleic Acid Purification 5x 50 rxns
MR-VI300 Viral Nucleic Acid Extraction Kit III Viral Nucleic Acid Purification Kits 300prep
MR-VI050 Viral Nucleic Acid Extraction Kit III Viral Nucleic Acid Purification Kits 50prep
MR-VR050 Viral Nucleic Acid Extraction Kit II Viral Nucleic Acid Purification Kits 50prep
MR-VI100 Viral Nucleic Acid Extraction Kit III Viral Nucleic Acid Purification Kits 100prep
MR-VR300 Viral Nucleic Acid Extraction Kit II Viral Nucleic Acid Purification Kits 300prep


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur