Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8611651
Publication Date : //

Arginine to tryptophan substitution in the active site of a human lactate dehydrogenase variant--LDHB GUA1: postulated effects on subunit structure and catalysis.


A variant of lactate dehydrogenase (LDHB GUA1) was previously identified among the Guaymi Indians of Panama and Costa Rica. The LDHB GUA1 variant is enzymatically inactive; however, the variant subunits alter the electrophoretic mobility of the tetramers that include active LDHA and LDHB subunits. The kinetic properties of the tetrameric enzyme, comprised of the inactive B plus active A subunits, are similar to properties of the heterotetramers with active B subunits, except for the reduced specific activity. We have determined that a single C.G to T.A transition changes an Arg to a Trp at amino acid residue 106. This substitution explains the increase in net negative charge observed by protein electrophoresis. This Arg 106 residue is absolutely conserved throughout evolution. Published high-resolution crystal structures of LDH reveal that this residue is within the hinge of a loop that closes over the active site of the subunit upon binding of substrate and cofactor and also has a direct role in catalysis. Computer modeling of the variant enzyme suggests that replacement of this Arg residue with a Trp does not induce significant change in the structure of the active site. However, this substitution would result in disruption of enzyme activity through the inability of the uncharged tryptophan side-chain to polarize the substrate carbonyl bond. This would explain the loss of the catalytic function with maintenance of normal kinetic properties in the heterotetramers containing the variant subunits. The ability to maintain normal, tissue-specific kinetic properties could explain the absence of clinical manifestations in the homozygous LDHB GUA1 individuals.

Authors : Shonnard G C , Hud N V , Mohrenweiser H W ,

Related products :

Catalog number Product name Quantity
orb82719 Human IL13 Variant protein Recombinant Human IL13 Variant is a single non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R (glutamine for arginine) at position 1 mg
DL-LDHB-Hu Human Lactate Dehydrogenase B (LDHB) ELISA Kit 96T
E2196h Homo sapiens,Human,LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Renal carcinoma antigen NY-REN-46
E2196p LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Pig,Sus scrofa
E2196h ELISA Homo sapiens,Human,LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Renal carcinoma antigen NY-REN-46 96T
E2196h ELISA kit Homo sapiens,Human,LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Renal carcinoma antigen NY-REN-46 96T
U2196h CLIA Homo sapiens,Human,LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Renal carcinoma antigen NY-REN-46 96T
U2196h CLIA kit Homo sapiens,Human,LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Renal carcinoma antigen NY-REN-46 96T
E2196p ELISA LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Pig,Sus scrofa 96T
U2196p CLIA kit LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Pig,Sus scrofa 96T
U2196p CLIA LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Pig,Sus scrofa 96T
E2196p ELISA kit LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Pig,Sus scrofa 96T
E2196Rb LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Oryctolagus cuniculus,Rabbit
E2196m LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Mouse,Mus musculus
E2196r LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Rat,Rattus norvegicus
E2196Rb ELISA LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Oryctolagus cuniculus,Rabbit 96T
E2196r ELISA LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Rat,Rattus norvegicus 96T
E2196m ELISA LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Mouse,Mus musculus 96T
E2196r ELISA kit LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Rat,Rattus norvegicus 96T
U2196r CLIA LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Rat,Rattus norvegicus 96T
E2196Rb ELISA kit LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Oryctolagus cuniculus,Rabbit 96T
U2196m CLIA kit LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Mouse,Mus musculus 96T
U2196r CLIA kit LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Rat,Rattus norvegicus 96T
U2196Rb CLIA kit LDH heart subunit,LDHB,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Oryctolagus cuniculus,Rabbit 96T
U2196m CLIA LDH heart subunit,Ldh2,Ldh-2,Ldhb,LDH-B,LDH-H,L-lactate dehydrogenase B chain,Mouse,Mus musculus 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur