Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9058381
Publication Date : //

Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta.


Estrogen receptor beta (ER beta) is a novel steroid receptor that is expressed in rat prostate and ovary. We have cloned the mouse homolog of ER beta and mapped the gene, designated Estrb, to the central region of chromosome 12. The cDNA encodes a protein of 485 amino acids that shares, respectively, 97% and 60% identity with the DNA- and ligand-binding domains of mouse (m) ER alpha. Mouse ER beta bind to an inverted repeat spaced by three nucleotides in a gel mobility shift assay and transactivates promoters containing synthetic or natural estrogen response elements in an estradiol (E2)-dependent manner. Scatchard analysis indicates that mER beta has slightly lower affinity for E2 [dissociation constant (Kd) = 0.5 nM] when compared with mER alpha (Kd = 0.2 nM). Antiestrogens, including 4-hydroxytamoxifen (OHT), ICI 182,780, and a novel compound, EM-800, inhibit E2-dependent transactivation efficiently. However, while OHT displays partial agonistic activity with ER alpha on a basal promoter linked to estrogen response elements in Cos-1 cells, this effect is not observed with mER beta. Cotransfection of mER beta and H-RasV12 causes enhanced activation in the presence of E2. Mutagenesis of a serine residue (position 60), located within a mitogen-activated protein kinase consensus phosphorylation site abolishes the stimulatory effect of Ras, suggesting that the activity of mER beta is also regulated by the mitogen-activated protein kinase pathway. Surprisingly, the coactivator SRC-1 up-regulates mER beta transactivation both in the absence and presence of E2, and in vitro interaction between SRC-1 and the ER beta ligand-binding domain is enhanced by E2. Moreover, the ligand-independent stimulatory effect of SRC-1 on ER beta transcriptional activity is abolished by ICI 182,780, but not by OHT. Our results demonstrate that while ER beta shares many of the functional characteristics of ER alpha, the molecular mechanisms regulating the transcriptional activity of mER beta may be distinct from those of ER alpha.

Authors : Tremblay G B , Tremblay A , Copeland N G , Gilbert D J , Jenkins N A , Labrie F , Giguère V ,

Related products :

Catalog number Product name Quantity
EIAAB13284 ERR beta-2,ERRB2,ERR-beta,ESRL2,ESRRB,Estrogen receptor-like 2,Estrogen-related receptor beta,Homo sapiens,Human,NR3B2,Nuclear receptor subfamily 3 group B member 2,Steroid hormone receptor ERR2
18-461-10247 Steroid hormone receptor ERR2 - Estrogen-related receptor. beta; ERR-beta; Estrogen receptor-like 2; ERR beta-2 Polyclonal 0.05 ml
18-461-10248 Steroid hormone receptor ERR2 - Estrogen-related receptor. beta; ERR-beta; Estrogen receptor-like 2; ERR beta-2 Polyclonal 0.05 ml
18-461-10249 Steroid hormone receptor ERR2 - Estrogen-related receptor. beta; ERR-beta; Estrogen receptor-like 2; ERR beta-2 Polyclonal 0.05 ml
18-003-42074 Steroid hormone receptor ERR2 - Estrogen-related receptor. beta; ERR-beta; Estrogen receptor-like 2; ERR beta-2 Polyclonal 0.05 mg Aff Pur
EIAAB13286 Err2,ERR-beta,Esrl2,Esrrb,Estrogen receptor-like 2,Estrogen-related receptor beta,Nr3b2,Nuclear receptor subfamily 3 group B member 2,Rat,Rattus norvegicus,Steroid hormone receptor ERR2
EIAAB13285 Err2,Err-2,ERR-beta,Esrrb,Estrogen receptor-like 2,Estrogen-related receptor beta,Mouse,Mus musculus,Nr3b2,Nuclear receptor subfamily 3 group B member 2,Steroid hormone receptor ERR2
MCA1974ST MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format Con S_N, Isotypes IgG2a, Applications 0.1 ml
SCH-MCA1974T MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format S_N, Isotypes IgG2a, Applications C, 0.2 ml
MCA1974T MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format S_N, Isotypes IgG2a, Applications C, 0.2 ml
SCH-MCA1974S MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format Con S_N, Isotypes IgG2a, Applications 1 ml
SCH-MCA1974 MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format S_N, Isotypes IgG2a, Applications C, 2 ml
MCA1974S MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format Con S_N, Isotypes IgG2a, Applications 1 ml
SCH-MCA1974ST MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format Con S_N, Isotypes IgG2a, Applications 0.1 ml
MCA1974 MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 1, Target Species Human, Host Mouse, Format S_N, Isotypes IgG2a, Applications C, 2 ml
SCH-MCA2279 MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 2, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 0.1 mg
SCH-MCA2279GT MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 2, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 50 µg
SCH-MCA4676T MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5 , Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 5, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 25 µg
MCA2279 MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 2, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 0.1 mg
MCA4676GA MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5 , Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 5, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 0.1 mg
MCA2279GT MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2, Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 2, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 50 µg
MCA4676T MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5 , Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 5, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 25 µg
SCH-MCA4676GA MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5 , Product Type Monoclonal Antibody, Specificity ESTROGEN RECEPTOR BETA 5, Target Species Human, Host Mouse, Format Purified, Isotypes IgG1, Applications 0.1 mg
20-272-192201 Estrogen Receptor beta - Mouse monoclonal to Estrogen Receptor beta; ER-beta Monoclonal 0.05 mg
E2300p ER-beta,ESR2,Estrogen receptor beta,NR3A2,Nuclear receptor subfamily 3 group A member 2,Pig,Sus scrofa


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur