Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9153233
Publication Date : //

Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation.


Nef is a 27-kDa myristoylated protein conserved in primate lentiviruses. In vivo, simian immunodeficiency virus Nef is required in macaques to produce a high viral load and full pathological effects. Nef has at least three major effects in vitro, induction of CD4 down-regulation, alteration of T cell activation pathways, and enhancement of viral infectivity. We have used the yeast two-hybrid system to identify cellular proteins that interact with HIV-1Lai Nef and could mediate Nef function. A human cDNA was isolated that encodes a new type of thioesterase, an enzyme that cleaves thioester bonds. This novel thioesterase is unlike the animal types I and II thioesterases previously cloned but is homologous to the Escherichia coli thioesterase II. Nef and this thioesterase interact in vitro and are co-immunoprecipitated by anti-Nef antibodies in CEM cells expressing Nef. Nef alleles from human immunodeficiency virus-1 (HIV-1) isolates unable to down-regulate CD4 do not react or react poorly with thioesterase. An HIV-1 NefLai mutant selected for its lack of interaction with thioesterase was also unable to down-regulate CD4 cell-surface expression. These observations suggest that this human thioesterase is a cellular mediator of Nef-induced CD4 down-regulation.

Authors : Liu L X , Margottin F , Le Gall S , Schwartz O , Selig L , Benarous R , Benichou S ,

Related products :

Catalog number Product name Quantity
27-751 The regulation of the quinone reductase (QR) gene as well as other genes involved in detoxification is known to be mediated by an electrophile response element (EpRE). QR gene regulation by the anties 0.05 mg
27-752 The regulation of the quinone reductase (QR) gene as well as other genes involved in detoxification is known to be mediated by an electrophile response element (EpRE). QR gene regulation by the anties 0.05 mg
29-058 The regulation of the quinone reductase (QR) gene as well as other genes involved in detoxification is known to be mediated by an electrophile response element (EpRE). QR gene regulation by the anties 0.1 mg
28-359 ZNF271 may be involved in transcriptional regulation. It plays a role in the EBV-mediated transformation process 0.05 mg
28-691 CSNK1g1 is involved in regulation of fast synaptic transmission mediated by glutamate, the major excitatory neurotransmitter in the brain. 0.1 mg
25-379 CSNK1g1 is involved in regulation of fast synaptic transmission mediated by glutamate, the major excitatory neurotransmitter in the brain. 0.05 mg
28-099 In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by RRN3, an essential initiation factor. It interacts with Pol I in the absence of template DNA, augments 0.1 mg
EIAAB37321 Mch,Olah,Oleoyl-ACP hydrolase,Rat,Rattus norvegicus,S-acyl fatty acid synthase thioesterase, medium chain,Thedc1,Thioesterase domain-containing protein 1,Thioesterase II
EIAAB37319 Mouse,Mus musculus,Olah,Oleoyl-ACP hydrolase,S-acyl fatty acid synthase thioesterase, medium chain,Thedc1,Thioesterase domain-containing protein 1,Thioesterase II
30-252 Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several 0.05 mg
30-952 C17orf81 belongs to the ELP5 family. C17orf81 may be involved in TP53-mediated transcriptional regulation. 0.05 mg
28-360 ZNF271 may be involved in transcriptional regulation. It plays a role in the EBV-mediated transformation processZinc finger proteins interact with nucleic acids and have diverse functions. The zinc fi 0.1 mg
EIAAB29345 C14orf137,Deubiquitinating enzyme OTUB2,Homo sapiens,Human,OTB2,OTU domain-containing ubiquitin aldehyde-binding protein 2,OTU2,OTUB2,Otubain-2,Ubiquitin thioesterase OTUB2,Ubiquitin-specific-processi
EIAAB29344 Deubiquitinating enzyme OTUB1,Homo sapiens,hOTU1,HSPC263,Human,OTB1,OTU domain-containing ubiquitin aldehyde-binding protein 1,OTU1,OTUB1,Otubain-1,Ubiquitin thioesterase OTUB1,Ubiquitin-specific-proc
EIAAB29343 Deubiquitinating enzyme OTUB1,Mouse,Mus musculus,OTU domain-containing ubiquitin aldehyde-binding protein 1,Otub1,Otubain-1,Ubiquitin thioesterase OTUB1,Ubiquitin-specific-processing protease OTUB1
EIAAB29342 Deubiquitinating enzyme OTUB1,OTU domain-containing ubiquitin aldehyde-binding protein 1,Otub1,Otubain-1,Rat,Rattus norvegicus,Ubiquitin thioesterase OTUB1,Ubiquitin-specific-processing protease OTUB1
EIAAB29346 Deubiquitinating enzyme OTUB2,Mouse,Mus musculus,OTU domain-containing ubiquitin aldehyde-binding protein 2,Otub2,Otubain-2,Ubiquitin thioesterase OTUB2,Ubiquitin-specific-processing protease OTUB2
27-922 MAFB is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The nuclear protein represses ETS1-mediated transcription o 0.05 mg
27-921 MAFB is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The nuclear protein represses ETS1-mediated transcription o 0.1 mg
30-302 PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation 0.05 mg
30-303 PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation 0.1 mg
29-789 This enzyme is probably necessary for target cell lysis in cell-mediated immune responses. 0.1 mg
30-940 GK2 belongs to the FGGY kinase family. GK2 is a key enzyme in the regulation of glycerol uptake and metabolism. 0.05 mg
25-668 Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation f 0.05 mg
27-148 TFPI2 may play a role in the regulation of plasmin-mediated matrix remodeling. It inhibits trypsin, plasmin, factor VIIa_tissue factor and weakly factor Xa. TFPI2 has no effect on thrombin. 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur