Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9218798
Publication Date : 1997/08/08

Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases.


A cDNA was cloned that encodes human stress-activated protein kinase-4 (SAPK4), a novel MAP kinase family member whose amino acid sequence is approximately 60% identical to that of the other three SAP kinases which contain a TGY motif in their activation domain. The mRNA encoding SAPK4 was found to be widely distributed in human tissues. When expressed in KB cells, SAPK4 was activated in response to cellular stresses and pro-inflammatory cytokines, in a manner similar to other SAPKs. SAPK4 was activated in vitro by SKK3 (also called MKK6) or when co-transfected with SKK3 into COS cells. SKK3 was the only activator of SAPK4 that was induced when KB cells were exposed to a cellular stress or stimulated with interleukin-1. These findings indicate that SKK3 mediates the activation of SAPK4. The substrate specificity of SAPK4 in vitro was similar to that of SAPK3. Both enzymes phosphorylated the transcription factors ATF2, Elk-1 and SAP-1 at similar rates, but were far less effective than SAPK2a (also called RK/p38) or SAPK2b (also called p38beta) in activating MAPKAP kinase-2 and MAPKAP kinase-3. Unlike SAPK1 (also called JNK), SAPK3 and SAPK4 did not phosphorylate the activation domain of c-Jun. Unlike SAPK2a and SAPK2b, SAPK4 and SAPK3 were not inhibited by the drugs SB 203580 and SB 202190. Our results suggest that cellular functions previously attributed to SAPK1 and/or SAPK2 may be mediated by SAPK3 or SAPK4.

Authors : Goedert M , Cuenda A , Craxton M , Jakes R , Cohen P ,

Related products :

Catalog number Product name Quantity
EIAAB24870 Homo sapiens,Human,MAP kinase 13,MAP kinase p38 delta,MAPK 13,MAPK13,Mitogen-activated protein kinase 13,Mitogen-activated protein kinase p38 delta,PRKM13,SAPK4,Stress-activated protein kinase 4
EIAAB24855 c-Jun N-terminal kinase 1,Homo sapiens,Human,JNK1,JNK-46,MAP kinase 8,MAPK 8,MAPK8,Mitogen-activated protein kinase 8,PRKM8,SAPK1,Stress-activated protein kinase 1,Stress-activated protein kinase JNK1
E02J0001 Rat c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
30-443 STK39 is a serine_threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several c 0.1 mg
30-444 STK39 is a serine_threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several c 0.05 mg
E07J0001 Porcine c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E03J0001 Mouse c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E14J0001 Sheep c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E08J0001 Canine c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E12J0001 Chicken c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
UB-E11596 Rat c-Jun N-terminal kinases per stress-activated protein kinase(JNK per SAPK)ELISA Kit 96T
E04J0001 Rabbit c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
YHB0229Ra Rat c-Jun N-terminal kinases-stress-activated protein kinase,JNK-SAPK ELISA Kit 96T
E11J0001 Bovine c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E09J0001 Monkey c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
YHB0229Ra Rat c-Jun N-terminal kinases-stress-activated protein kinase,JNK-SAPK ELISA Kit 48T
E0048Ra Rat c-Jun N-terminal kinases-stress-activated protein kinase,JNK-SAPK ELISA Kit 96T
E05J0001 Guinea Pig c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E01J0001 Human c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E06J0001 Goat c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E0048Ra Rat c-Jun N-terminal kinases per stress-activated protein kinase,JNK per SAPK ELISA Kit 96T
E0047Ra Rat c-Jun N-terminal kinases-stress-activated protein kinase,JNK-SAPK ELISA Kit 48T
E02P0752 Rat Phosphorylated c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit
E0048Ra Rat c-Jun N-terminal kinases per stress-activated protein kinase,JNK per SAPK ELISA Kit 48T
E07P0752 Porcine Phosphorylated c-Jun N-terminal kinases stress-activated protein kinase ELISA 96T/kit


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur