Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9732274
Publication Date : //

Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome.


The Escherichia coli RNA degradosome is the prototype of a recently discovered family of multiprotein machines involved in the processing and degradation of RNA. The interactions between the various protein components of the RNA degradosome were investigated by Far Western blotting, the yeast two-hybrid assay, and coimmunopurification experiments. Our results demonstrate that the carboxy-terminal half (CTH) of ribonuclease E (RNase E) contains the binding sites for the three other major degradosomal components, the DEAD-box RNA helicase RhlB, enolase, and polynucleotide phosphorylase (PNPase). The CTH of RNase E acts as the scaffold of the complex upon which the other degradosomal components are assembled. Regions for oligomerization were detected in the amino-terminal and central regions of RNase E. Furthermore, polypeptides derived from the highly charged region of RNase E, containing the RhlB binding site, stimulate RhlB activity at least 15-fold, saturating at one polypeptide per RhlB molecule. A model for the regulation of the RhlB RNA helicase activity is presented. The description of RNase E now emerging is that of a remarkably complex multidomain protein containing an amino-terminal catalytic domain, a central RNA-binding domain, and carboxy-terminal binding sites for the other major components of the RNA degradosome.

Authors : Vanzo N F , Li Y S , Py B , Blum E , Higgins C F , Raynal L C , Krisch H M , Carpousis A J ,

Related products :

Catalog number Product name Quantity
30-250 Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta tran 0.1 mg
26-175 Neuroplastin is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta tran 0.05 mg
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur