Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9791011
Publication Date : //

Molecular cloning of rat klotho cDNA: markedly decreased expression of klotho by acute inflammatory stress.


We have recently identified a novel gene, termed klotho, that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the beta-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human Klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho.

Authors : Ohyama Y , Kurabayashi M , Masuda H , Nakamura T , Aihara Y , Kaname T , Suga T , Arai M , Aizawa H , Matsumura Y , Kuro-o M , Nabeshima Y i , Nagail R ,

Related products :

Catalog number Product name Quantity
enz-369 Recombinant Human Klotho KLOTHO 5
enz-369 Recombinant Human Klotho KLOTHO 0.5mg
enz-369 Recombinant Human Klotho KLOTHO 20
orb91430 Human Klotho beta gene Human Klotho beta gene is a cDNA gene. For research use only. 10
IC-1007 pEco-CMV-cHis-(Neo) PCR cloning kit: PCR cloning kit with a built-in mammalian expression vector (with Neomycin selection marker) in provided cloning cells, for mammalian expression of C-term His-tagg 10 x 50ul/ea
ASC-ASH-0017 Molecular cloning reagents, RT-PCR reagent: SuperRT cDNA Kit 25 rxn
ASC-ASH-0017 Molecular cloning reagents, RT-PCR reagent: SuperRT cDNA Kit 25 rxn
ASC-ASH-0018 Molecular cloning reagents, RT-PCR reagent: HiFi-MMLV cDNA Kit 25 rxn
ASC-ASH-0018 Molecular cloning reagents, RT-PCR reagent: HiFi-MMLV cDNA Kit 25 rxn
IC-1001 pEco-T7-nHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein. 10 x 50ul/ea
IC-1004 pEco-T7-nGST PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term GST-tagged protein. 10 x 50ul/ea
IC-1006 pEco-T7-cHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells, for E Coli expression of C-term His-tagged protein. 10 x 50ul/ea
IC-1003 pEco-BAD-nHis PCR cloning kit: PCR cloning kit with a built-in vector (non-T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein, specially designed for toxic 10 x 50ul/ea
CNB-1437518 Human Klotho beta full length cDNA gene clone _ ORF gene clone 10
PB511B-1 PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector 10 ug
PB512B-1 PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector 10 ug
IC-1002 pEco-suCMV-nHis-(Neo) PCR cloning kit: PCR cloning kit with a built-in mammalian expression vector (with neomycin selection marker) in provided cloning cells. The vector containing an engineered super 10 x 50ul/ea
DL-KL-Ra Rat Klotho (KL) ELISA Kit 96T
Y214240 Klotho _ KL 200ul
Y212329 Klotho 200ul
EB07011 Klotho 0.1 mg
DM3583P Klotho 0.1 mg
ARP41554_P050 Klotho 50 µg
ARP41552_P050 Klotho 50 µg
AR08005PU-S Klotho 5 µg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur