Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :10537218
Publication Date : //

Nucleosides as a carbon source in Bacillus subtilis: characterization of the drm-pupG operon.


In Bacillus subtilis, nucleosides are readily taken up from the growth medium and metabolized. The key enzymes in nucleoside catabolism are nucleoside phosphorylases, phosphopentomutase, and deoxyriboaldolase. The characterization of two closely linked loci, drm and pupG, which encode phosphopentomutase (Drm) and guanosine (inosine) phosphorylase (PupG), respectively, is reported here. When expressed in Escherichia coli mutant backgrounds, drm and pupG confer phosphopentomutase and purine-nucleoside phosphorylase activity. Northern blot and enzyme analyses showed that drm and pupG form a dicistronic operon. Both enzymes are induced when nucleosides are present in the growth medium. Using mutants deficient in nucleoside catabolism, it was demonstrated that the low-molecular-mass effectors of this induction most likely were deoxyribose 5-phosphate and ribose 5-phosphate. Both Drm and PupG activity levels were higher when succinate rather than glucose served as the carbon source, indicating that the expression of the operon is subject to catabolite repression. Primer extension analysis identified two transcription initiation signals upstream of drm; both were utilized in induced and non-induced cells. The nucleoside-catabolizing system in B. subtilis serves to utilize the base for nucleotide synthesis while the pentose moiety serves as the carbon source. When added alone, inosine barely supports growth of B. subtilis. This slow nucleoside catabolism contrasts with that of E. coli, which grows rapidly on a nucleoside as a carbon source. When inosine was added with succinate or deoxyribose, however, a significant increase in growth was observed in B. subtilis. The findings of this study therefore indicate that the B. subtilis system for nucleoside catabolism differs greatly from the well-studied system in E. coli.

Authors : Schuch R , Garibian A , Saxild H H , Piggot P J , Nygaard P ,

Related products :

Catalog number Product name Quantity
9074-98-0 beta_Glucanase from bacillus subtilis, 1 from bacillus subtilis, 1 1g
A30960 a_Amylase (Bacillus Subtilis source) >10 units_mg 100 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units mg 100 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units per mg 500 G
A30960 a_Amylase (Bacillus Subtilis source) >10 units_mg 500 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units mg 500 G
A30960 a_Amylase (Bacillus Subtilis source) >10 units_mg 25 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units mg 25 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units per mg 25 G
A30960 a-Amylase (Bacillus Subtilis source) >10 units per mg 100 G
B452 Carbon Utilisation Agar (ISP Medium No.9) USE : For characterization of Streptomyces on the basis of carbon utilization. 5x500gm
B563 ISP Medium No.9 (Carbon Utilisation Agar) USE : For characterization of Streptomyces on the basis of carbon utilization. 5x500gm
B452 Carbon Utilisation Agar (ISP Medium No.9) USE For characterization of Streptomyces on the basis of carbon utilization. Qty per Litre of Medium: 26,67
B563 ISP Medium No.9 (Carbon Utilisation Agar) USE For characterization of Streptomyces on the basis of carbon utilization. Qty per Litre of Medium: 26,67
B1140 Bacillus Differentiation Agar USE For differentiation between Bacillus cereus and Bacillus subtilis based on mannitol fermentation. Qty per Litre of Medium: 22
B1140 Bacillus Differentiation Agar USE : For differentiation between Bacillus cereus and Bacillus subtilis based on mannitol fermentation. 5x500gm
B606 Maintenance Medium For B.Subtilis ATCC 6633 USE For maintenance of Bacillus subtilis ATCC 6633,used for microbiological assay of antibiotics. Qty per Litre of Medium: 30,5
B606 Maintenance Medium For B.Subtilis ATCC 6633 USE : For maintenance of Bacillus subtilis ATCC 6633,used for microbiological assay of antibiotics. 5x500gm
TM 1242 MAINTENANCE MEDIUM FOR B.SUBTILIS ATCC 6633 (for maintenance of Bacillus subtilis ATCC 6633 as the test organism for as 100 gm
TM 1242 MAINTENANCE MEDIUM FOR B.SUBTILIS ATCC 6633 (for maintenance of Bacillus subtilis ATCC 6633 as the test organism for as 100 gm
9001-47-2 Glutaminase from bacillus subtilis 50 ug 1g
9014-01-1 Subtilisin ex Bacillus subtilis 1g
0801562 Bacillus subtilis Z094, titered 1 mL
GEN0801562DNA-10UG Bacillus subtilis Z094, DNA (10 EA
NE150/PAb rabbit PAb against Bacillus subtilis Subtilisin 1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur