Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11003841
Publication Date : //

MES-1, a protein required for unequal divisions of the germline in early C. elegans embryos, resembles receptor tyrosine kinases and is localized to the boundary between the germline and gut cells.


During Caenorhabditis elegans embryogenesis the primordial germ cell, P(4), is generated via a series of unequal divisions. These divisions produce germline blastomeres (P(1), P(2), P(3), P(4)) that differ from their somatic sisters in their size, fate and cytoplasmic content (e.g. germ granules). mes-1 mutant embryos display the striking phenotype of transformation of P(4) into a muscle precursor, like its somatic sister. A loss of polarity in P(2) and P(3) cell-specific events underlies the Mes-1 phenotype. In mes-1 embryos, P(2) and P(3) undergo symmetric divisions and partition germ granules to both daughters. This paper shows that mes-1 encodes a receptor tyrosine kinase-like protein, though it lacks several residues conserved in all kinases and therefore is predicted not to have kinase activity. Immunolocalization analysis shows that MES-1 is present in four- to 24-cell embryos, where it is localized in a crescent at the junction between the germline cell and its neighboring gut cell. This is the region of P(2) and P(3) to which the spindle and P granules must move to ensure normal division asymmetry and cytoplasmic partitioning. Indeed, during early stages of mitosis in P(2) and P(3), one centrosome is positioned adjacent to the MES-1 crescent. Staining of isolated blastomeres demonstrated that MES-1 was present in the membrane of the germline blastomeres, consistent with a cell-autonomous function. Analysis of MES-1 distribution in various cell-fate and patterning mutants suggests that its localization is not dependent on the correct fate of either the germline or the gut blastomere but is dependent upon correct spatial organization of the embryo. Our results suggest that MES-1 directly positions the developing mitotic spindle and its associated P granules within P(2) and P(3), or provides an orientation signal for P(2)- and P(3)-specific events.

Authors : Berkowitz L A , Strome S ,

Related products :

Catalog number Product name Quantity
ASC-ASM-5006 ESC-SureTM Germline Transmission Tested fetal bovine serum, Ideal for the purpose of generating knock-out_in mice, tested for germline transmission of mouse ES cells. 100 mL
ASC-ASM-5007 ESC-SureTM Germline Transmission Tested fetal bovine serum, Ideal for the purpose of generating knock-out_in mice, tested for germline transmission of mouse ES cells. 500 mL
ASC-ASM-5007 ESC-SureTM Germline Transmission Tested fetal bovine serum, Ideal for the purpose of generating knock-out_in mice, tested for germline transmission of mouse ES cells. 500 mL
ASC-ASM-5006 ESC-SureTM Germline Transmission Tested fetal bovine serum, Ideal for the purpose of generating knock-out_in mice, tested for germline transmission of mouse ES cells. 100 mL
ASC-ASE-9005 GermLine mESC C57BL6_129SvJ : Mouse embryonic stem cells derived from mouse embryos of C57BL6 and 129SvJ F1 hybrid strain ^1x10^6 cells/vial
ASC-ASE-9005 GermLine mESC C57BL6_129SvJ : Mouse embryonic stem cells derived from mouse embryos of C57BL6 and 129SvJ F1 hybrid strain ^1x10^6 cells/vial
ASC-ASE-9008 GermLine BALB_c-EZ Mouse Embryonic Stem Cells ^2.5X10^6 cells/vial
ASC-ASE-9008 GermLine BALB_c-EZ Mouse Embryonic Stem Cells ^2.5X10^6 cells/vial
25-840 CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, wh 0.05 mg
32-118 BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be i 0.1 mL
32-117 BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be i 0.1 mL
32-116 BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be i 0.1 mL
32-232 Tyk2 (tyrosine kinase 2),with 1187-amino acid protein (about 131kDa), belongs to the family of non-receptor janus tyrosine kinases, which also includes Jak1, Jak2, and Jak3. Kinases of the Jak family 0.1 mL
ASE-9006 GermLine mESC 129-EZ ^2.5X10^6 cells/vial
ASE-9006 GermLine mESC 129-EZ 1 vial of ^2.5 x10^6 cells
ASE-9007 GermLine mESC C57BL_6-EZ ^2.5X10^6 cells/vial
ASE-9008 GermLine mESC BALB_c EZ ^2.5X10^6 cells/vial
ASE-9005 GermLine mESC C57BL6_129SvJ ^1x10^6 cells/vial
ASE-9007 GermLine mESC C57BL per 6-EZ 1 vial of ^2.5 x10^6 cells
ASE-9008 GermLine mESC BALB c EZ 1 vial of ^2.5 x10^6 cells
ASE-9008 GermLine mESC BALB per c EZ 1 vial of ^2.5 x10^6 cells
ASE-9007 GermLine mESC C57BL 6-EZ 1 vial of ^2.5 x10^6 cells
ASC-6032 FBS evaluation FBS Test for Germline Service
ASE-9005 GermLine mESC C57BL6 129SvJ 1 vial of 1 x 10^6 cells
ASE-9005 GermLine mESC C57BL6 per 129SvJ 1 vial of 1 x 10^6 cells


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur