Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11463832
Publication Date : //

Negative regulation of protein translation by mitogen-activated protein kinase-interacting kinases 1 and 2.


Eukaryotic initiation factor 4E (eIF4E) is a key component of the translational machinery and an important modulator of cell growth and proliferation. The activity of eIF4E is thought to be regulated by interaction with inhibitory binding proteins (4E-BPs) and phosphorylation by mitogen-activated protein (MAP) kinase-interacting kinase (MNK) on Ser209 in response to mitogens and cellular stress. Here we demonstrate that phosphorylation of eIF4E via MNK1 is mediated via the activation of either the Erk or p38 pathway. We further show that expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. The same effect on cap-dependent translation was observed when MNK1 was activated by the Erk or p38 pathway. In line with these findings, addition of recombinant active MNK1 to rabbit reticulocyte lysate resulted in a reduced protein synthesis in vitro, and overexpression of MNK2 caused a decreased rate of protein synthesis in 293 cells. By using CGP 57380, a novel low-molecular-weight kinase inhibitor of MNK1, we demonstrate that eIF4E phosphorylation is not crucial to the formation of the initiation complex, mitogen-stimulated increase in cap-dependent translation, and cell proliferation. Our results imply that activation of MNK by MAP kinase pathways does not constitute a positive regulatory mechanism to cap-dependent translation. Instead, we propose that the kinase activity of MNKs, eventually through phosphorylation of eIF4E, may serve to limit cap-dependent translation under physiological conditions.

Authors : Knauf U , Tschopp C , Gram H ,

Related products :

Catalog number Product name Quantity
EIAAB38485 Homo sapiens,Human,MAPKAP1,MIP1,Mitogen-activated protein kinase 2-associated protein 1,mSIN1,SAPK-interacting protein 1,SIN1,Stress-activated map kinase-interacting protein 1,Target of rapamycin comp
EIAAB38489 Bos taurus,Bovine,MAPKAP1,Mitogen-activated protein kinase 2-associated protein 1,SAPK-interacting protein 1,SIN1,Stress-activated map kinase-interacting protein 1,Target of rapamycin complex 2 subuni
EIAAB38487 Mapkap1,Mip1,Mitogen-activated protein kinase 2-associated protein 1,Rat,Rattus norvegicus,SAPK-interacting protein 1,Sin1,Stress-activated map kinase-interacting protein 1,Target of rapamycin complex
EIAAB38486 Mapkap1,Mip1,Mitogen-activated protein kinase 2-associated protein 1,Mouse,Mus musculus,SAPK-interacting protein 1,Sin1,Stress-activated map kinase-interacting protein 1,Target of rapamycin complex 2
EIAAB41234 Homo sapiens,Human,MAP3K7IP3,Mitogen-activated protein kinase kinase kinase 7-interacting protein 3,NF-kappa-B-activating protein 1,TAB3,TAB-3,TAK1-binding protein 3,TGF-beta-activated kinase 1 and MA
EIAAB38488 Chicken,Gallus gallus,MAPKAP1,Mitogen-activated protein kinase 2-associated protein 1,SAPK-interacting protein 1,SIN1,Stress-activated map kinase-interacting protein 1,Target of rapamycin complex 2 su
EIAAB41230 Homo sapiens,Human,KIAA0733,MAP3K7IP2,Mitogen-activated protein kinase kinase kinase 7-interacting protein 2,TAB2,TAB-2,TAK1-binding protein 2,TGF-beta-activated kinase 1 and MAP3K7-binding protein 2,
EIAAB41233 Kiaa4135,Map3k7ip3,Mitogen-activated protein kinase kinase kinase 7-interacting protein 3,Mouse,Mus musculus,Tab3,TAB-3,TAK1-binding protein 3,TGF-beta-activated kinase 1 and MAP3K7-binding protein 3,
EIAAB41231 Kiaa0733,Map3k7ip2,Mitogen-activated protein kinase kinase kinase 7-interacting protein 2,Mouse,Mus musculus,Tab2,TAB-2,TAK1-binding protein 2,TGF-beta-activated kinase 1 and MAP3K7-binding protein 2,
EIAAB41232 Map3k7ip2,Mitogen-activated protein kinase kinase kinase 7-interacting protein 2,Rat,Rattus norvegicus,Tab2,TGF-beta-activated kinase 1 and MAP3K7-binding protein 2,TGF-beta-activated kinase 1-binding
EIAAB41228 Homo sapiens,Human,MAP3K7IP1,Mitogen-activated protein kinase kinase kinase 7-interacting protein 1,TAB1,TAK1-binding protein 1,TGF-beta-activated kinase 1 and MAP3K7-binding protein 1,TGF-beta-activa
EIAAB24864 Homo sapiens,Human,MAP kinase 11,MAP kinase p38 beta,MAPK 11,MAPK11,Mitogen-activated protein kinase 11,Mitogen-activated protein kinase p38 beta,p38-2,p38b,PRKM11,SAPK2,Stress-activated protein kinas
10-782-55059 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.005 mg
10-782-55059 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.02 mg
10-782-55060 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.05 mg
10-782-55060 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.02 mg
10-782-55059 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.001 mg
10-782-55059 Mitogen-activated protein kinase 12 - EC 2.7.11.24; Extracellular signal-regulated kinase 6; ERK-6; ERK5; Stress-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 0.01 mg
EIAAB24870 Homo sapiens,Human,MAP kinase 13,MAP kinase p38 delta,MAPK 13,MAPK13,Mitogen-activated protein kinase 13,Mitogen-activated protein kinase p38 delta,PRKM13,SAPK4,Stress-activated protein kinase 4
EIAAB41229 Map3k7ip1,Mitogen-activated protein kinase kinase kinase 7-interacting protein 1,Mouse,Mus musculus,Tab1,TAK1-binding protein 1,TGF-beta-activated kinase 1 and MAP3K7-binding protein 1,TGF-beta-activa
EIAAB24831 Mapk1ip1l,MAPK-interacting and spindle-stabilizing protein-like,Mitogen-activated protein kinase 1-interacting protein 1-like,Mouse,Mus musculus
EIAAB24833 Bos taurus,Bovine,MAPK1IP1L,MAPK-interacting and spindle-stabilizing protein-like,Mitogen-activated protein kinase 1-interacting protein 1-like
EIAAB24830 Mapk1ip1,MAPK-interacting and spindle-stabilizing protein,Miss,Mitogen-activated protein kinase 1-interacting protein 1,Mouse,Mus musculus
EIAAB24832 C14orf32,Homo sapiens,Human,MAPK1IP1L,MAPK-interacting and spindle-stabilizing protein-like,Mitogen-activated protein kinase 1-interacting protein 1-like
18-661-15208 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 - TGF-beta-activated kinase 1-binding protein 1; TAK1-binding protein 1 Polyclonal 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur