Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11672424
Publication Date : //

Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases.


GSH-dependent prostaglandin D(2) synthase (PGDS) enzymes represent the only vertebrate members of class Sigma glutathione S-transferases (GSTs) identified to date. Complementary DNA clones encoding the orthologous human and rat GSH-dependent PGDS (hPGDS and rPGDS, respectively) have been expressed in Escherichia coli, and the recombinant proteins isolated by affinity chromatography. The purified enzymes were both shown to catalyse specifically the isomerization of prostaglandin (PG) H(2) to PGD(2). Each transferase also exhibited GSH-conjugating and GSH-peroxidase activities. The ability of hPGDS to catalyse the conjugation of aryl halides and isothiocyanates with GSH was found to be less than that of the rat enzyme. Whilst there is no difference between the enzymes with respect to their K(m) values for 1-chloro-2,4-dinitrobenzene, marked differences were found to exist with respect to their K(m) for GSH (8 mM versus 0.3 mM for hPGDS and rPGDS, respectively). Using molecular modelling techniques, amino acid substitutions have been identified in the N-terminal domain of these enzymes that lie outside the proposed GSH-binding site, which may explain these catalytic differences. The tissue-specific expression of PGDS also varies significantly between human and rat; amongst the tissues examined, variation in expression between the two species was most apparent in spleen and bone marrow. Differences in catalytic properties and tissue-specific expression of hPGDS and rPGDS appears to reflect distinct physiological roles for class Sigma GST between species. The evolution of divergent functions for the hPGDS and rPGDS is discussed in the context of the orthologous enzyme from chicken.

Authors : Jowsey I R , Thomson A M , Flanagan J U , Murdock P R , Moore G B , Meyer D J , Murphy G J , Smith S A , Hayes J D ,

Related products :

Catalog number Product name Quantity
29-649 At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified alpha, kappa, mu, omega, pi, sigma, theta and zeta. GSTM2 is a glutathione S-tr 0.05 mg
26-383 The omega class glutathione transferases (GST; EC 2.5.1.18) have poor activity with common GST substrates, but exhibit novel glutathione-dependent thioltransferase, dehydroascorbate reductase, and mon 0.05 mg
29-276 The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected b 0.05 mg
29-642 Cytosolic and membrane-bound forms of glutathione S-transferase are two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases 0.05 mg
29-813 Members of the claudin protein family, such as CLDN2, are expressed in an organ-specific manner and regulate the tissue-specific physiologic properties of tight junctions.Members of the claudin protei 0.1 mg
EIAAB31926 Calcineurin, testis-specific catalytic subunit,Calmodulin-dependent calcineurin A subunit gamma isoform,CALNA3,CAM-PRP catalytic subunit,CNA3,Homo sapiens,Human,PPP3CC,Serine_threonine-protein phospha
AS03 031 mPGES_1 glutathione dependent prostaglandin E synthase 100 ul
AS03031 mPGES_1 glutathione dependent prostaglandin E synthase 100 ul
Y100023 mPGES_1 glutathione dependent prostaglandin E synthase 100ul
Y100023 mPGES-1 glutathione dependent prostaglandin E synthase Antibody 100ul
AS03 031 Antibody: mPGES-1 glutathione dependent prostaglandin E synthase , Host: rabbit, polyclonal, Comfirmed reactivity: human, mouse, rat, sheep 100
28-779 Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH protei 0.1 mg
25-056 Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH protei 0.05 mg
AS03 031 rabbit polyclonal mPGES-1 glutathione dependent prostaglandin E synthase 100
AS03031S mPGES_1 glutathione dependent prostaglandin E synthase protein | positive control 50 ul (20.5 ug)
AS03 031S mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control 50
30-446 PGDS is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells 0.05 mg
EIAAB31927 Calcineurin, testis-specific catalytic subunit,Calmodulin-dependent calcineurin A subunit gamma isoform,Calnc,CAM-PRP catalytic subunit,Mouse,Mus musculus,Ppp3cc,Serine_threonine-protein phosphatase 2
IC-1007 pEco-CMV-cHis-(Neo) PCR cloning kit: PCR cloning kit with a built-in mammalian expression vector (with Neomycin selection marker) in provided cloning cells, for mammalian expression of C-term His-tagg 10 x 50ul/ea
E01G0374 Human Glutathione S Transferases 96 Tests/kit
E01α0008 Human α-glutathione S-transferases 96 Tests/kit
E01A0767 Human a-glutathione S-transferases 96 Tests/kit
E01G0374 Human Anti-Glutathione S Transferases 96 Tests/kit
E01G0374 Human Glutathione S Transferases ELISA 96T/kit
201-12-1506 Human α-glutathione S-transferases,α-GST ELISA Kit 48T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur