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Pubmed ID :11752432
Publication Date : //

Partial purification and identification of GDP-mannose 3",5"-epimerase of Arabidopsis thaliana, a key enzyme of the plant vitamin C pathway.


The first step in the biosynthetic pathway of vitamin C in plants is the formation, at the level of sugar nucleotide, of l-galactosyl residues, catalyzed by a largely unknown GDP-d-mannose 3",5"-epimerase. By using combined conventional biochemical and mass spectrometry methods, we obtained a highly purified preparation of GDP-d-mannose 3",5"-epimerase from an Arabidopsis thaliana cell suspension. The native enzyme is an 84-kDa dimer, composed of two apparently identical subunits. In-gel tryptic digestion of the enzyme subunit, followed by peptide sequencing and a blast search, led to the identification of the epimerase gene. The closest homolog of the plant epimerase is the BlmG gene product of Streptomyces sp., a putative NDP-d-mannose 5"-epimerase. The plant GDP-d-mannose 3",5"-epimerase is, to our knowledge, a novel member of the extended short-chain dehydrogenase/reductase family. The enzyme was cloned and expressed in Escherichia coli cells.

Authors : Wolucka B A , Persiau G , Van Doorsselaere J , Davey M W , Demol H , Vandekerckhove J , Van Montagu M , Zabeau M , Boerjan W ,

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Catalog number Product name Quantity
GWB-ECFF87 Arabidopsis thaliana Plant Lysate
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AS09 477 Antibody: S14 | 40S ribosomal protein S14-1, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 476 Antibody: AtArf2 | ADP ribosylation factor 2, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1783 Antibody: Acd1 | accelerated cell death 1 , Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 474 Antibody: AtMTP4 | metal tolerance protein B, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 515 Antibody: KC1 | potassium channel KAT3 , Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 674 Antibody: Ferritin (plant), Immunogen: KLH-conjugated synthetic peptide derived from known plant ferritin sequences including Arabidopsis thaliana Q39101, Host: rabbit, polyclonal, Confirmed reactivit 200
AS09 475 Antibody: Ligand-gated ion channel 1.3, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Raph 100
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
AS10 651 Antibody: Enolase, Immunogen: recombinant Arabidopsis thaliana enolase At2g36530, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 671 Antibody: AGO5 | argonaute 5, Immunogen: peptide of Arabidopsis thaliana AGO5 Q9SJK3, AT2G27880, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 937 Antibody: CPLD11 , Immunogen: overexpressed fragment of recombinant Arabidopsis thaliana CPLD11 NP_566650, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 672 Antibody: AGO6 | argonaute 6, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 At2g32940, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 459 Antibody: ClpB-P | ClpB3, Immunogen: KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS06 116 Antibody: PGL35 | plastoglobulin 35, Immunogen: recombinant Arabidopsis thaliana PGL35 protein O81439 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Gossypium hirsutum cv. Del 200
AS10 720 Antibody: PHOT1 | phototropin-1, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
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AS10 721 Antibody: PHOT2 | phototropin-2, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1628 Antibody: HSP18.5 | class IV heat shock protein, Immunogen: recombinant Arabidopsis thaliana Hsp18.5 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
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AS11 1629 Antibody: HSP90-2 | heat shock protein 90-2, Immunogen: full length recombinant Hsp90-2 from Arabidopsis thaliana, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS05 059 Antibody: PsbR | 10 kDa protein of PSII, Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsbR P27202, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, 100
AS09 481 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 200
AS08 346 Antibody: HSP90-1 | heat shock protein 90-1, Immunogen: full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Zea m 100


 

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