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Pubmed ID :11753363
Publication Date : //

An integrated approach for finding overlooked genes in yeast.


We report here the discovery of 137 previously unappreciated genes in yeast through a widely applicable and highly scalable approach integrating methods of gene-trapping, microarray-based expression analysis, and genome-wide homology searching. Our approach is a multistep process in which expressed sequences are first trapped using a modified transposon that produces protein fusions to beta-galactosidase (beta-gal); non-annotated open reading frames (ORFs) translated as beta-gal chimeras are selected as a candidate pool of potential genes. To verify expression of these sequences, labeled RNA is hybridized against a microarray of oligonucleotides designed to detect gene transcripts in a strand-specific manner. In complement to this experimental method, novel genes are also identified in silico by homology to previously annotated proteins. As these methods are capable of identifying both short ORFs and antisense ORFs, our approach provides an effective supplement to current gene-finding schemes. In total, the genes discovered using this approach constitute 2% of the yeast genome and represent a wealth of overlooked biology.

Authors : Kumar Anuj , Harrison Paul M , Cheung Kei-Hoi , Lan Ning , Echols Nathaniel , Bertone Paul , Miller Perry , Gerstein Mark B , Snyder Michael ,

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SRP0104 This plate consists of 65 siRNAs targeting human Receptor genes (Mouse homolog 53 genes). 1 Plate
SRP0040 This plate consists of 80 siRNAs targeting human Kinase genes (Mouse homolog 76 genes). 1 Plate
SRP0103 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 65 genes). 1 Plate
SRP0101 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 47 genes). 1 Plate
SRP0100 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 40 genes). 1 Plate
SRP0099 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 47 genes). 1 Plate
SRP0105 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 77 genes). 1 Plate
SRP0106 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 78 genes). 1 Plate
SRP0041 This plate consists of 80 siRNAs targeting human Kinase genes (Mouse homolog 77 genes). 1 Plate
SRP0043 This plate consists of 80 siRNAs targeting human Kinase genes (Mouse homolog 76 genes). 1 Plate
SRP0044 This plate consists of 80 siRNAs targeting human Kinase genes (Mouse homolog 78 genes). 1 Plate
SRP0110 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 70 genes). 1 Plate
SRP0042 This plate consists of 80 siRNAs targeting human Kinase genes (Mouse homolog 79 genes). 1 Plate
SRP0109 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 68 genes). 1 Plate
SRP0108 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 62 genes). 1 Plate
SRP0107 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 67 genes). 1 Plate
SRP0098 This plate consists of 80 siRNAs targeting human Receptor genes (Mouse homolog 62 genes). 1 Plate


 

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