Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :12920053
Publication Date : //

Purification and characterization of PLUNC from human tracheobronchial secretions.


To study proteins secreted into the airway, we used secretions from primary human airway epithelial cells, re-differentiated at the air-liquid interface, and from patients intubated during surgery. A major protein of the cultured cell secretions was ethanol soluble. This protein was purified, analyzed by Edman degradation, matrix-assisted laser-desorption ionization time-of-flight mass spectroscopy of tryptic digests, and Western blots of two-dimensional electrophoresis gels using antisera against the purified preparation. The protein was identified as palate, lung, nasal epithelium clone protein (PLUNC). The protein had multiple truncated molecules, a pattern also seen in tracheal aspirates. PLUNC was poorly soluble in water (50 microg/ml) or in 50 mM NaCl but was more soluble in 75% ethanol (> 380 microg/ml). PLUNC secretion dramatically increased during the second week in air-liquid interface culture and continued to increase over time. Immunohistochemistry showed that PLUNC was expressed in human airway epithelium and submucosal glands. Although PLUNC is in the lipopolysaccharide (LPS)-binding protein (LBP) and bactericidal/permeability-increasing protein family of antibacterial host defense proteins, purified PLUNC failed to compete with LBP for the binding of LPS, whereas polymyxin B, a known inhibitor of LPS-LBP binding, did interfere with binding. This study showed that plunc gene product is expressed both in vivo and in vitro, detailed a method for its purification and provided basic information on its biochemical properties in secretions.

Authors : Campos Michael A , Abreu Alexandre R , Nlend Marie C , Cobas Miguel A , Conner Gregory E , Whitney Philip L ,

Related products :

Catalog number Product name Quantity
DL-PLUNC-Hu Human Palate-Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit 96T
DL-PLUNC-Hu Human Palate_Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit 96T
20-783-72954 MOUSE ANTI HUMAN MUCIN 5AC - MUC-5AC; Mucin-5 subtype AC. tracheobronchial; Tracheobronchial mucin; TBM; Major airway glycoprotein; Gastric mucin; Lewis B blood group antigen; LeB Monoclonal 0.2 mg
29-758 PLUNC is the human homolog of murine plunc and is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this protein is not known, however, it has be 0.05 mg
E0756h ELISA kit Gastric mucin,Homo sapiens,Human,LeB,Lewis B blood group antigen,Major airway glycoprotein,MUC5,MUC5AC,MUC-5AC,Mucin-5 subtype AC, tracheobronchial,Mucin-5AC,TBM,Tracheobronchial mucin 96T
U0756h CLIA Gastric mucin,Homo sapiens,Human,LeB,Lewis B blood group antigen,Major airway glycoprotein,MUC5,MUC5AC,MUC-5AC,Mucin-5 subtype AC, tracheobronchial,Mucin-5AC,TBM,Tracheobronchial mucin 96T
E0756h ELISA Gastric mucin,Homo sapiens,Human,LeB,Lewis B blood group antigen,Major airway glycoprotein,MUC5,MUC5AC,MUC-5AC,Mucin-5 subtype AC, tracheobronchial,Mucin-5AC,TBM,Tracheobronchial mucin 96T
DL-PLUNC-Mu Mouse Palate-Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit 96T
DL-PLUNC-Mu Mouse Palate_Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit 96T
CSB-EL018213MO Mouse Protein Plunc(PLUNC) ELISA kit SpeciesMouse 96T
CSB-EL018213PI Pig Protein Plunc(PLUNC) ELISA kit SpeciesPig 96T
G3962 Protein Plunc (PLUNC), Mouse, ELISA Kit 96T
CSB-EL018213RA Rat Protein Plunc(PLUNC) ELISA kit SpeciesRat 96T
CSB-EL018213MO Mouse Protein Plunc(PLUNC) ELISA kit 96T
CSB-EL018213RA Rat Protein Plunc(PLUNC) ELISA kit 96T
G3964 Protein Plunc (PLUNC), Rat, ELISA Kit 96T
G3963 Protein Plunc (PLUNC), Pig, ELISA Kit 96T
20-272-191614 Mucin 5AC. prediluted - Mouse monoclonal [SPM297] to Mucin 5AC. prediluted; Mucin-5 subtype AC. tracheobronchial; Tracheobronchial mucin; TBM; Major airway glycoprotein Monoclonal 7 ml
DL-PLUNC-Hu PLUNC ELISA kit 96T
DL-PLUNC-Mu PLUNC ELISA kit 96T
H00051297-P01-25 PLUNC (Human) Recombinant Protein (P01) 25 ug
DC03439 Human PLUNC cDNA Clone
Y054529 Purified Rabbit Anti Human PLUNC Polyclonal 100μl
DC03439 Human PLUNC cDNA Clone, Vector pcDNA4_TO_myc-His A 10 ug
LS-B3549-50 PLUNC Rabbit anti-Human Polyclonal Antibody


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur