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Pubmed ID :14500720
Publication Date : //

Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.


Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.

Authors : Takagi Yuichiro , Komori Hirofumi , Chang Wei-Hau , Hudmon Andy , Erdjument-Bromage Hediye , Tempst Paul , Kornberg Roger D ,

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