Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :14562106
Publication Date : //

Global analysis of protein expression in yeast.


The availability of complete genomic sequences and technologies that allow comprehensive analysis of global expression profiles of messenger RNA have greatly expanded our ability to monitor the internal state of a cell. Yet biological systems ultimately need to be explained in terms of the activity, regulation and modification of proteins--and the ubiquitous occurrence of post-transcriptional regulation makes mRNA an imperfect proxy for such information. To facilitate global protein analyses, we have created a Saccharomyces cerevisiae fusion library where each open reading frame is tagged with a high-affinity epitope and expressed from its natural chromosomal location. Through immunodetection of the common tag, we obtain a census of proteins expressed during log-phase growth and measurements of their absolute levels. We find that about 80% of the proteome is expressed during normal growth conditions, and, using additional sequence information, we systematically identify misannotated genes. The abundance of proteins ranges from fewer than 50 to more than 10(6) molecules per cell. Many of these molecules, including essential proteins and most transcription factors, are present at levels that are not readily detectable by other proteomic techniques nor predictable by mRNA levels or codon bias measurements.

Authors : Ghaemmaghami Sina , Huh Won-Ki , Bower Kiowa , Howson Russell W , Belle Archana , Dephoure Noah , O'Shea Erin K , Weissman Jonathan S ,

Related products :

Catalog number Product name Quantity
28-057 CNOT3 is a protein component of CCR4-NOT protein complex. Yeast CCR$-NOT is a global regulator of RNA polymerase II transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional prot 0.1 mg
28-056 CNOT3 is a protein component of CCR4-NOT protein complex. Yeast CCR$-NOT is a global regulator of RNA polymerase II transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional prot 0.05 mg
YT207 Yeast cracking buffer stock solution for protein expression test,Yeast transformation reagent 5x100 ml
V-1010-V6 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VC155-KanMX6
V-1010-V1 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VN173-HIS3MX6
V-1010-V2 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VC155-HIS3MX6
V-1010-V3 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VN173-TRP1
V-1010-V4 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VC155-TRP1
V-1010-V5 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-VN173-KanMX6
V-1010-V7 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-HIS3MX6-PGAL1-VN173
V-1010-V10 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-TRP1-PGAL1-VC155
V-1010-V12 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-KanMX6-PGAL1-VC155
V-1010-V9 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-TRP1-PGAL1-VN173
V-1010-V13 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-HIS3MX6-PCET1-VN173
V-1010-V14 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-HIS3MX6-PCET1-VC155
V-1010-V8 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-HIS3MX6-PGAL1-VC155
V-1010-V17 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-KanMX6-PCET1-VN173
V-1010-V18 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-KanMX6-PCET1-VC155
V-1010-V16 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-TRP1-PCET1-VC155
V-1010-V15 Yeast protein tagging vectors for BiFC analysis 5ug (Air-dried) pFA6a-TRP1-PCET1-VN173
30-652 Over expression of PDCD2L attenutates TNF-alpha release in Daudi cells. PDCD2L over-expression restrained proliferation of HEK293T cells. DNA_flow cytometry analysis showed that the over-expression of 0.05 mg
22120006-1 Protein expression in yeast
27-441 LASS2 is a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein m 0.05 mg
25-078 LASS2 is a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein m 0.05 mg
B585 M-BCG Yeast And Mold Broth USE : For the detection of fungi in routine analysis of beverages using membrane filter technique. 5x500gm


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur