Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :15299904
Publication Date : //

Enhanced diffractivity of phosphoglucomutase crystals. Use of an alternative cryocrystallographic procedure.


A continuous procedure for replacing the 2 M (NH(4))(2)SO(4) within crystals of rabbit muscle phosphoglucomutase by 55% polyethylene glycol 600 (PEG 600) is described. The success rate (absence of fracturing) approaches 100% in spite of the fragility of the crystals. The procedure is based on the use of a biphasic PEG/salt mixture in conjunction with a flow cell that allows several crystals to be treated at once. The holdup volume of the cell is small and its design minimizes concentration gradients. Cooling treated crystals to 253 K elicits a substantial increase in diffractivity that allows data collection to be extended from about 2.75 to about 2.35 A. However, neither the dimensions of the unit cell nor the structure of the asymmetric unit are significantly altered. Comparisons are made between data sets collected at 289 K using crystals in 2.2 M (NH(4))(2)SO(4) and at 253 K using crystals in 55% PEG 600. Models of the enzyme refined against one or the other data sets are also compared. These comparisons suggest that, at most, only a minor fraction of the increased diffractivity is caused by lowered atomic B values. The increased diffractivity also is not the result of a simple temperature effect since at high-salt concentration the same cooling protocol fails to significantly increase diffractivity. A decrease in mosaic spread is considered as a possible explanation for the increased diffractivity.

Authors : Ray W J , Baranidharan S , Liu Y ,

Related products :

Catalog number Product name Quantity
950-20 L(+)LACTIC ACID - Ultra Pure Crystals Small Crystals 25 g
950-20 L(+)LACTIC ACID - Ultra Pure Crystals Small Crystals 100 g
950-20 L(+)LACTIC ACID - Ultra Pure Crystals Small Crystals 1000 g
950-20 L(+)LACTIC ACID - Ultra Pure Crystals Small Crystals 5 g
EKIN-400 EnzyChrom™ Kinase Assay Kit, Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm_590nm) method. Procedure 1 400tests
EIAAB30738 Aciculin,Homo sapiens,Human,PGM5,PGMRP,PGM-RP,Phosphoglucomutase-like protein 5,Phosphoglucomutase-related protein
orb90505 Enhanced chemiluminescence kit (Rat IgG) Enhanced chemiluminescence kit (Rat IgG) for Western Blotting For research use only. 1 kit
2216-51-5 L_Menthol, crystals natural crystals natural 1g
SL100309 Western Blotting Reagents, LumiGOLD™ Enhanced Chemiluminescent Western Blotting Detection Kit, an enhanced highly sensitive substrate for detecting horseradish peroxidase (HRP) on immunoblots. This su 250 ml
SL100309 Western Blotting Reagents, LumiGOLD™ Enhanced Chemiluminescent Western Blotting Detection Kit, an enhanced highly sensitive substrate for detecting horseradish peroxidase (HRP) on immunoblots. This su 250 ml
SL100309-L Western Blotting Reagents, LumiGOLD™ Enhanced Chemiluminescent Western Blotting Detection Kit, an enhanced highly sensitive substrate for detecting horseradish peroxidase (HRP) on immunoblots. This su 1000 ml
SL100309-L Western Blotting Reagents, LumiGOLD™ Enhanced Chemiluminescent Western Blotting Detection Kit, an enhanced highly sensitive substrate for detecting horseradish peroxidase (HRP) on immunoblots. This su 1000 ml
AS10 699 Antibody: AOX | alternative oxidase, Immunogen: alternative oxidase purified from Sauromatum guttatum (the voodoo lily), Host: mouse, monoclonal, Confirmed reactivity: Arabidopsis thaliana, Glycine ma 1,5 ml
orb90502 Enhanced chemiluminescence kit (Mouse IgG) Enhanced chemiluminescence kit (Mouse IgG) for Western Blotting For research use only. 1 kit
orb90506 Enhanced chemiluminescence kit (Mouse IgM) Enhanced chemiluminescence kit (Mouse IgM) for Western Blotting For research use only. 1 kit
orb90503 Enhanced chemiluminescence kit (Rabbit IgG) Enhanced chemiluminescence kit (Rabbit IgG) for Western Blotting For research use only. 1 kit
orb90504 Enhanced chemiluminescence kit (Goat IgG) Enhanced chemiluminescence kit (Goat IgG) for Western Blotting For research use only. 1 kit
TM 301 THIOGLYCOLLATE BROTH, ALTERNATIVE (ALTERNATIVE THIOGLYCOLLATE MEDIUM) (as per USP) 100 gm
TM 301 THIOGLYCOLLATE BROTH, ALTERNATIVE (ALTERNATIVE THIOGLYCOLLATE MEDIUM) (as per USP) 500 gm
D02190 Desoxyribonuclease crystals 10 MG
D02190 Desoxyribonuclease crystals
77-68-1 TRIS Pre-set crystals Reagent pH 8.1 +_ - 1g
A-00848 AMMONIUM SULPHITE CRYSTALS 500 gm
M28644860 TRIS Pre-set crystals Reagent pH 8.1 +_- 0.05 CAS: [77-86-1] 500 g
KMD40 Tooth preparation procedure,


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur