GENTAUR antibody-antibodies.com The Marketplace for Antibodies : The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :15673572
Publication Date : //

The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.

The homeobox gene Mixl1 is expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. The role of Mixl1 in development of haematopoietic mesoderm was investigated by analysing the differentiation of ES cells in which GFP was targeted to one (Mixl1(GFP/w)) or both (Mixl1(GFP/GFP)) alleles of the Mixl1 locus. In either case, GFP was transiently expressed, with over 80% of cells in day 4 embryoid bodies (EBs) being GFP(+). Up to 45% of Mixl1(GFP/w) day 4 EB cells co-expressed GFP and the haemangioblast marker FLK1, and this doubly-positive population was enriched for blast colony forming cells (BL-CFCs). Mixl1-null ES cells, however, displayed a haematopoietic defect characterised by reduced and delayed Flk1 expression and a decrease in the frequency of haematopoietic CFCs. These data indicated that Mixl1 was required for efficient differentiation of cells from the primitive streak stage to blood. Differentiation of ES cells under serum-free conditions demonstrated that induction of Mixl1- and Flk1-expressing haematopoietic mesoderm required medium supplemented with BMP4 or activin A. In conclusion, this study has revealed an important role for Mixl1 in haematopoietic development and demonstrates the utility of the Mixl1(GFP/w) ES cells for evaluating growth factors influencing mesendodermal differentiation.

Authors : Ng Elizabeth S , Azzola Lisa , Sourris Koula , Robb Lorraine , Stanley Edouard G , Elefanty Andrew G ,

Related products :

Catalog number	Product name	Quantity

29-969	SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and	0.05 mg
25-503	MESP1 is a transcription factor. MESP1 plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. MESP1 defines the rostrocaudal patterning of the somites by	0.05 mg
29-882	AS an endosomal ferrireductase, STEAP3 is required for efficient transferrin-dependent iron uptake in erythroid cells. It participates in erythroid iron homeostasis by reducing Fe (3+) to Fe (2+) and	0.1 mg
29-876	AS an endosomal ferrireductase, STEAP3 is required for efficient transferrin-dependent iron uptake in erythroid cells. It participates in erythroid iron homeostasis by reducing Fe (3+) to Fe (2+) and	0.05 mg
27-934	CRSP9 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP9 is also a component of other multisubunit com	0.05 mg
MIXL1_MOUSE	ELISA Kit FOR Homeobox protein MIXL1; organism: Mouse; gene name: Mixl1	96T
27-282	MIXL1 is a novel human Mix-like homeobox gene. In normal hematopoiesis, its expression appears to be restricted to immature B- and T-lymphoid cells.	0.05 mg
26-525	DBX1 could have a role in patterning the central nervous system during embryogenesis. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral i	0.05 mg
28-881	TCF15 is found in the nucleus and may be involved in the early transcriptional regulation of patterning of the mesoderm. The basic helix-loop-helix protein requires dimerization with another basic hel	0.1 mg
26-412	DOLPP1 is a multi-pass membrane proteinBy similarity. It belongs to the dolichyldiphosphatase family. It is required for efficient N-glycosylation and is necessary for maintaining optimal levels of do	0.05 mg
EIAAB27565	Homo sapiens,Human,Neuro-oncological ventral antigen 1,NOVA1,Onconeural ventral antigen 1,Paraneoplastic Ri antigen,RNA-binding protein Nova-1,Ventral neuron-specific protein 1
28-645	TSG101 has a direct role in the control of growth and differentiation in primary epithelial cells. It is required for normal cell function of embryonic and adult tissues but that this gene is not a tu	0.1 mg
28-758	TSG101 has a direct role in the control of growth and differentiation in primary epithelial cells. It is required for normal cell function of embryonic and adult tissues but that this gene is not a tu	0.1 mg
CSB-EL014596MO	Mouse Homeobox protein MIXL1(MIXL1) ELISA kit SpeciesMouse	96T
CSB-EL014596HU	Human Homeobox protein MIXL1(MIXL1) ELISA kit SpeciesHuman	96T
CSB-EL014596MO	Mouse Homeobox protein MIXL1(MIXL1) ELISA kit	96T
CSB-EL014596HU	Human Homeobox protein MIXL1(MIXL1) ELISA kit	96T
A0425	BMP4 Polyclonal Antibody, Reactivity: H, Gene ID: 652, Synonyms: BMP4	200ug
A0425	BMP4 Polyclonal Antibody, Reactivity: H, Gene ID: 652, Synonyms: BMP4	100ug
A1565	BMP4 Polyclonal Antibody, Reactivity: H M R, Gene ID: 652, Synonyms: BMP4	100ug
A1565	BMP4 Polyclonal Antibody, Reactivity: H M R, Gene ID: 652, Synonyms: BMP4	200ug
A0424	BMP4 Polyclonal Antibody, Reactivity: H M R, Gene ID: 652, Synonyms: BMP4	100ug
A0424	BMP4 Polyclonal Antibody, Reactivity: H M R, Gene ID: 652, Synonyms: BMP4	200ug
28-339	Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the	0.1 mg
P-5006-BMP4-96	QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation _ BMP4 96 assays	96 assays

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF