Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :15973356
Publication Date : //

TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing.


MicroRNAs (miRNAs) are generated by a two-step processing pathway to yield RNA molecules of approximately 22 nucleotides that negatively regulate target gene expression at the post-transcriptional level. Primary miRNAs are processed to precursor miRNAs (pre-miRNAs) by the Microprocessor complex. These pre-miRNAs are cleaved by the RNase III Dicer to generate mature miRNAs that direct the RNA-induced silencing complex (RISC) to messenger RNAs with complementary sequence. Here we show that TRBP (the human immunodeficiency virus transactivating response RNA-binding protein), which contains three double-stranded, RNA-binding domains, is an integral component of a Dicer-containing complex. Biochemical analysis of TRBP-containing complexes revealed the association of Dicer-TRBP with Argonaute 2 (Ago2), the catalytic engine of RISC. The physical association of Dicer-TRBP and Ago2 was confirmed after the isolation of the ternary complex using Flag-tagged Ago2 cell lines. In vitro reconstitution assays demonstrated that TRBP is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. Knockdown of TRBP results in destabilization of Dicer and a consequent loss of miRNA biogenesis. Finally, depletion of the Dicer-TRBP complex via exogenously introduced siRNAs diminished RISC-mediated reporter gene silencing. These results support a role of the Dicer-TRBP complex not only in miRNA processing but also as a platform for RISC assembly.

Authors : Chendrimada Thimmaiah P , Gregory Richard I , Kumaraswamy Easwari , Norman Jessica , Cooch Neil , Nishikura Kazuko , Shiekhattar Ramin ,

Related products :

Catalog number Product name Quantity
011-22033 Anti AGO2 Human, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 50 ul
018-22021 Anti AGO2 Mouse, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 100 ul
014-22023 Anti AGO2 Mouse, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 50 ul
015-22031 Anti AGO2 Human, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 100 ul
292-66701 microRNA Isolation kit, Human Ago2 microRNA Isolation kit, Human Ago2 10 reactions
orb92594 Mouse AGO2 gene Mouse AGO2 gene is a cDNA gene. For research use only. 10
25-655 TNRC6A is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The 0.05 mg
292-66701 microRNA Isolation kit, Human Ago2 10 reactions
A4138 TRBP Primary Antibody, TRBP, Species: Human Recombinant Protein Source: Rabbit Polyclonal 100ug
292-66701 microRNA Isolation kit, Human Ago2 General Reagents 10 reactions
292-66701 microRNA Isolation kit, Human Ago2 General Reagents siRNA_RNA Transcription 10 reactions
25-644 EXOSC2 belongs to the exosome, a RNA-processing complex, which is at least involved in the 3' processing of the 7S pre-rRNA to the mature 5.8S rRNA. It exhibits a 3'-5' exoribonuclease activity. 0.05 mg
29-419 EXOSC2 belongs to the exosome, a RNA-processing complex, which is at least involved in the 3' processing of the 7S pre-rRNA to the mature 5.8S rRNA. It exhibits a 3'-5' exoribonuclease activity. 0.1 mg
LF-MA0209 anti-TRBP (46D1), Mouse monoclonal to TRBP, Isotype IgG1, Host Mouse 100 ul
E0156b ELISA Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
U0156b CLIA Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
E0156b ELISA kit Bos taurus,Bovine,DICER,DICER1,Endoribonuclease Dicer 96T
27-306 Fadd is apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) per 0.05 mg
25-409 GTF2E2 belongs to the TFIIE beta subunit family.It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. 0.05 mg
31-027 This is a receptor for the cytotoxic ligand TNFSF10_TRAIL. The adaptor molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspas 0.1 mg
27-249 GTF2E1 belongs to the TFIIE alpha subunit family. It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH 0.05 mg
25-643 The signal recognition particle (SRP) is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six p 0.05 mg
3697BP-50 Dicer Blocking Peptide target: Dicer 50 μg
3697BP-50 Dicer Blocking Peptide Dicer 50 μg
26-127 RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 pr 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur