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Pubmed ID :16246292
Publication Date : //

A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein.


The investigation of hormone-receptor interaction normally needs isolation and extensive purification of the receptor protein or a particular receptor-containing fraction. To bypass these time- and resource-consuming procedures, we have established a live cell-based assay using transgenic bacteria expressing single eukaryotic receptors. Here we describe some biochemical features of the Arabidopsis cytokinin receptor CRE1/AHK4 expressed in Escherichia coli. The data show that the main characteristics of the ligand-receptor interaction, including binding affinity and ligand specificity, can be determined using intact bacteria expressing a functional receptor.

Authors : Romanov Georgy A , Spíchal Lukás , Lomin Sergey N , Strnad Miroslav , Schmülling Thomas ,

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