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Pubmed ID :16847258
Publication Date : //

A global topology map of the Saccharomyces cerevisiae membrane proteome.


The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.

Authors : Kim Hyun , Melén Karin , Osterberg Marie , von Heijne Gunnar ,

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