Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :16936728
Publication Date : //

Involvement of AGO1 and AGO2 in mammalian transcriptional silencing.


Duplex RNAs complementary to messenger RNA inhibit translation in mammalian cells by RNA interference (RNAi). Studies have reported that RNAs complementary to promoter DNA also inhibit gene expression. Here we show that the human homologs of Argonaute-1 (AGO1) and Argonaute-2 (AGO2) link the silencing pathways that target mRNA with pathways mediating recognition of DNA. We find that synthetic antigene RNAs (agRNAs) complementary to transcription start sites or more upstream regions of gene promoters inhibit gene transcription. This silencing occurs in the nucleus, requires high promoter activity and does not necessarily require histone modification. AGO1 and AGO2 associate with promoter DNA in cells treated with agRNAs, and inhibiting expression of AGO1 or AGO2 reverses transcriptional and post-transcriptional silencing. Our data indicate key linkages and important mechanistic distinctions between transcriptional and post-transcriptional silencing pathways in mammalian cells.

Authors : Janowski Bethany A , Huffman Kenneth E , Schwartz Jacob C , Ram Rosalyn , Nordsell Robert , Shames David S , Minna John D , Corey David R ,

Related products :

Catalog number Product name Quantity
011-22033 Anti AGO2 Human, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 50 ul
015-22031 Anti AGO2 Human, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 100 ul
014-22023 Anti AGO2 Mouse, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 50 ul
018-22021 Anti AGO2 Mouse, Monoclonal Antibody Argonaute_2(AGO2)was isolated as one of the main components of RISC (RNA_induced silencing complex). AGO2 captures siRNAand microRNA which are working as a guide m 100 ul
28-385 VGLL1 is a specific coactivator for the mammalian TEFs. The mammalian TEF and the Drosophila scalloped genes belong to a conserved family of transcriptional factors that possesses a TEA_ATTS DNA-bindi 0.1 mg
25-655 TNRC6A is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The 0.05 mg
AS09 527P Antibody: AGO1 | argonaute 1 | Blocking peptide, Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379 100
29-063 NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing tran 0.1 mg
31-192 NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing tran 0.05 mg
29-062 NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing tran 0.1 mg
AS09 527 Antibody: AGO1 | argonaute 1, Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana 100
27-258 Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subun 0.05 mg
31-193 Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subun 0.1 mg
27-242 Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subun 0.05 mg
10-288-22393F Cofactor required for Sp1 transcriptional activation subunit 9 - Transcriptional coactivator CRSP33; RNA polymerase transcriptional regulation mediator subunit 7 homolog; hMED7; Activator-recruited co 0.05 mg
18-003-43107 Cofactor required for Sp1 transcriptional activation subunit 9 - Transcriptional coactivator CRSP33; RNA polymerase transcriptional regulation mediator subunit 7 homolog; hMED7; Activator-recruited co 0.05 mg Aff Pur
10-288-22393F Cofactor required for Sp1 transcriptional activation subunit 9 - Transcriptional coactivator CRSP33; RNA polymerase transcriptional regulation mediator subunit 7 homolog; hMED7; Activator-recruited co 0.1 mg
orb83538 Human AGO1 His tag protein Recombinant Human AGO1 His tag protein. For research use only. 50
25-501 ZNF415 is involved in transcriptional regulation. The transcriptional activity differed among the various isoforms. All isoforms except isoform 3 seem to suppress the transcriptional activities of AP- 0.05 mg
29-051 ZNF415 is involved in transcriptional regulation. The transcriptional activity differed among the various isoforms. All isoforms except isoform 3 seem to suppress the transcriptional activities of AP- 0.05 mg
orb83400 Human AGO2 His tag protein Recombinant Human AGO2 His tag protein. For research use only. 20
orb84447 Mouse AGO2 His tag protein Recombinant Mouse AGO2 His tag protein. For research use only. 20
EIAAB37309 Charged amino acid-rich leucine zipper 1,Crlz1,Disrupter of silencing SAS10,Rat,Rattus norvegicus,Sas10,Something about silencing protein 10,Utp3,UTP3 homolog
EIAAB37307 Charged amino acid-rich leucine zipper 1,Crl1,Crl-1,Crlz1,Disrupter of silencing SAS10,Mouse,Mus musculus,Sas10,Something about silencing protein 10,Utp3,UTP3 homolog
EIAAB37308 Charged amino acid-rich leucine zipper 1,CRL1,CRLZ1,Disrupter of silencing SAS10,Homo sapiens,Human,SAS10,Something about silencing protein 10,UTP3,UTP3 homolog


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur