Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :17495932
Publication Date : //

Disulphide-isomerase-enabled shedding of tumour-associated NKG2D ligands.


Tumour-associated ligands of the activating NKG2D (natural killer group 2, member D; also called KLRK1) receptor-which are induced by genotoxic or cellular stress-trigger activation of natural killer cells and co-stimulation of effector T cells, and may thus promote resistance to cancer. However, many progressing tumours in humans counter this anti-tumour activity by shedding the soluble major histocompatibility complex class-I-related ligand MICA, which induces internalization and degradation of NKG2D and stimulates population expansions of normally rare NKG2D+CD4+ T cells with negative regulatory functions. Here we show that on the surface of tumour cells, MICA associates with endoplasmic reticulum protein 5 (ERp5; also called PDIA6 or P5), which, similar to protein disulphide isomerase, usually assists in the folding of nascent proteins inside cells. Pharmacological inhibition of thioreductase activity and ERp5 gene silencing revealed that cell-surface ERp5 function is required for MICA shedding. ERp5 and membrane-anchored MICA form transitory mixed disulphide complexes from which soluble MICA is released after proteolytic cleavage near the cell membrane. Reduction of the seemingly inaccessible disulphide bond in the membrane-proximal alpha3 domain of MICA must involve a large conformational change that enables proteolytic cleavage. These results uncover a molecular mechanism whereby domain-specific deconstruction regulates MICA protein shedding, thereby promoting tumour immune evasion, and identify surface ERp5 as a strategic target for therapeutic intervention.

Authors : Kaiser Brett K , Yim Daesong , Chow I-Ting , Gonzalez Segundo , Dai Zhenpeng , Mann Henning H , Strong Roland K , Groh Veronika , Spies Thomas ,

Related products :

Catalog number Product name Quantity
E02N0038 Rat soluble NKG2D ligands ELISA 96T/kit
E12N0038 Chicken soluble NKG2D ligands ELISA 96T/kit
E06N0038 Goat soluble NKG2D ligands ELISA 96T/kit
E08N0038 Canine soluble NKG2D ligands ELISA 96T/kit
E04N0038 Rabbit soluble NKG2D ligands ELISA 96T/kit
E05N0038 Guinea Pig soluble NKG2D ligands ELISA 96T/kit
E01N0038 Human soluble NKG2D ligands ELISA 96T/kit
E07N0038 Porcine soluble NKG2D ligands ELISA 96T/kit
E14N0038 Sheep soluble NKG2D ligands ELISA 96T/kit
E09N0038 Monkey soluble NKG2D ligands ELISA 96T/kit
E11N0038 Bovine soluble NKG2D ligands ELISA 96T/kit
E03N0038 Mouse soluble NKG2D ligands ELISA 96T/kit
MC-027 Protein Disulphide Isomerase 200 uL
MC-027 Protein Disulphide Isomerase 200 uL
MC-027 Protein Disulphide Isomerase 200 uL
AM12077PU-N Protein Disulphide Isomerase 0.2 ml
MC-027 Protein Disulphide Isomerase 38H8 200 uL
MC-027 Protein Disulphide Isomerase Clone 38H8 200 uL
MC-027 Antibodies: Protein Disulphide Isomerase Clone: 38H8 200 uL
MAB3350 Protein Disulphide Isomerase monoclonal antibody, clone 38H8 100 uL
NKG2D-101AP Natural Killer Group 2 MemberD (NKG2D) antibodies WB control: PC-NKG2D Host: Rabbit Affinity purifed 100-150ul
NKG2D-101AP Natural Killer Group 2 MemberD (NKG2D) antibodies Antigenic peptide: P-NKG2D Host: Rabbit Affinity purifed 100ul
18-783-78984 GOAT ANTI PDIA3 (C-TERMINAL) - PROTEIN DISULPHIDE ISOMERASE FAMILY A MEMBER 3 Polyclonal 0.1 mg
NKG2D-101AP Natural Killer Group 2 MemberD (NKG2D) antibodies FITC-conjugates: NKG2D-FITC Host: Rabbit Affinity purifed 200ul
NKG2D-101AP Natural Killer Group 2 MemberD (NKG2D) antibodies Biotin Conjugates: NKG2D-Biotin Host: Rabbit Affinity purifed 200ul


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur