Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :19154332
Publication Date : //

Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease.


Ribosomal RNAs (rRNAs) are processed from larger primary transcripts in every living system known. The maturation of 23S rRNA in Bacillus subtilis is catalysed by Mini-III, a member of the RNase III family of enzymes that lacks the characteristic double-stranded RNA binding domain of its relatives. We have previously shown that Mini-III processing of 23S precursor rRNA in assembled 50S ribosomal subunits is much more efficient than a substrate with no ribosomal proteins bound, suggesting that one or more large subunit proteins act as a cofactor for Mini-III cleavage. Here we show that this cofactor is ribosomal protein L3. Stimulation of the Mini-III cleavage reaction is through L3 binding to its normal site at the 3' end of 23S rRNA. We present indirect evidence that suggests that L3 acts at the level of substrate, rather than enzyme conformation. We also discuss the potential implication of using ribosomal protein cofactors in rRNA processing for ribosome quality control.

Authors : Redko Yulia , Condon Ciarán ,

Related products :

Catalog number Product name Quantity
EIAAB26943 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1,18S rRNA Psi1248 methyltransferase,C2F,EMG1,Homo sapiens,Human,Nucleolar protein EMG1 homolog,Protein C2f,Ribosomal RNA small subunit methyltransfer
E0447r Mouse ELISA Kit FOR rRNA maturation factor homolog 96T
E1864m Human ELISA Kit FOR rRNA maturation factor homolog 96T
SH21B_HUMAN Mouse ELISA Kit FOR rRNA maturation factor homolog 96T
G8514 RRNA maturation factor homolog (C21orf57), Human, ELISA Kit 96T
EIAAB46623 Mouse,Mus musculus,rRNA maturation factor homolog,Ybey
CSB-EL003773HU Human rRNA maturation factor homolog(C21orf57) ELISA kit 96T
CSB-EL003773HU Human rRNA maturation factor homolog(C21orf57) ELISA kit SpeciesHuman 96T
YBEY_MOUSE ELISA Kit FOR rRNA maturation factor homolog; organism: Mouse; gene name: Ybey 96T
EIAAB46622 C21orf57,Homo sapiens,Human,rRNA maturation factor homolog,YBEY
orb80699 Adrenocorticotropic Hormone protein Adrenocorticotropic hormone, as its name implies, stimulates the adrenal cortex. More specifically, it stimulates secretion of glucocorticoids such as cortisol, and 2 mg
NB100-662 ribosomal RNA (rRNA 5.8s) 0.1 ml
11020001-3 rRNA (Ribosomal RNA) 5 mg
11020001-2 rRNA (Ribosomal RNA) 2 mg
11020001-1 rRNA (Ribosomal RNA) 1 mg
NB100-662 Ribosomal RNA (rRNA 5.8s) 0.1 ml
26-794 EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of e 0.05 mg
EIAAB35973 Ftsj2,Mouse,Mus musculus,Protein ftsJ homolog 2,Putative ribosomal RNA methyltransferase 2,rRNA (uridine-2'-O-)-methyltransferase
EIAAB35974 FJH1,FTSJ2,Homo sapiens,Human,Protein ftsJ homolog 2,Putative ribosomal RNA methyltransferase 2,rRNA (uridine-2'-O-)-methyltransferase
EIAAB35972 FTSJ1,Homo sapiens,Human,JM23,Protein ftsJ homolog 1,Putative ribosomal RNA methyltransferase 1,rRNA (uridine-2'-O-)-methyltransferase
EIAAB35975 Ftsj3,Protein ftsJ homolog 3,Putative rRNA methyltransferase 3,Rat,Rattus norvegicus,rRNA (uridine-2'-O-)-methyltransferase 3
EIAAB35978 Ftsj3,Mouse,Mus musculus,Protein ftsJ homolog 3,Putative rRNA methyltransferase 3,rRNA (uridine-2'-O-)-methyltransferase 3
EIAAB35977 FTSJ3,Homo sapiens,Human,Protein ftsJ homolog 3,Putative rRNA methyltransferase 3,rRNA (uridine-2'-O-)-methyltransferase 3,SB92
EIAAB36248 37 kDa laminin receptor precursor,37_67 kDa laminin receptor,37LRP,40S ribosomal protein SA,67 kDa laminin receptor,67LR,Laminin receptor 1,Laminin-binding protein precursor p40,LamR,Lamr1,LBP_p40,LRP
EIAAB36246 37 kDa laminin receptor precursor,37_67 kDa laminin receptor,37LRP,40S ribosomal protein SA,67 kDa laminin receptor,67LR,Laminin receptor 1,Laminin-binding protein precursor p40,LamR,LAMR1,LBP_p40,LRP


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur