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Pubmed ID :19423640
Publication Date : //

Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs.


Alternative splicing (AS) is a mechanism by which multiple types of mature mRNAs are generated from a single pre-mature mRNA. In this study, we completely sequenced 1800 full-length cDNAs from Arabidopsis thaliana, which had 5' and/or 3' sequences that were previously found to have AS events or alternative transcription start sites. Unexpectedly, these sequences gave us further evidence of AS, as 601 out of 1800 transcripts showed novel AS events. We focused on the combination patterns of multiple AS events within individual genes. Interestingly, some specific AS event combination patterns tended to appear more frequently than expected. The two most common patterns were: (i) alternative donor-0 approximately 12 times of exon skips-alternative acceptor and (ii) several times ( approximately 8) of retained introns. We also found that multiple AS events in a transcript tend to have the same effects concerning the length of the mature mRNA. Our current results are consistent with our previous observations, which showed changes in AS profiles under different conditions, and suggest the involvement of hypothetical cis- and trans-acting factors in the regulation of AS events.

Authors : Iida Kei , Fukami-Kobayashi Kaoru , Toyoda Atsushi , Sakaki Yoshiyuki , Kobayashi Masatomo , Seki Motoaki , Shinozaki Kazuo ,

Related products :

Catalog number Product name Quantity
AS08 346 Antibody: HSP90-1 | heat shock protein 90-1, Immunogen: full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Zea m 100
AS11 1629 Antibody: HSP90-2 | heat shock protein 90-2, Immunogen: full length recombinant Hsp90-2 from Arabidopsis thaliana, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS10 702 Antibody: Actin-11, Immunogen: full length ACT11 (Actin-11) from Arabidopsis thaliana, accession number NP_187818.1, Host: mouse, monoclonal, Confirmed reactivity: Arabidopsis thaliana, Nicotiana taba 100
AS11 1633 Antibody: EF1A | elongation factor 1-alpha _ EF-1-alpha, Immunogen: full length, recombinant EF1A of Arabidopsis thaliana P13905, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS08 325 Antibody: Arf1 | ADP-ribosylation factor 1, Immunogen: recombinant GST fusion of full length of Arabidopsis thaliana ARF1 At2g47170, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thalian 200
AS09 647 Antibody: GSNOR | S-nitrosoglutathione reductase, Immunogen: overexpressed, full length GSNOR derived from Arabidopsis thaliana Q96533, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thal 200
AS10 699 Antibody: AOX | alternative oxidase, Immunogen: alternative oxidase purified from Sauromatum guttatum (the voodoo lily), Host: mouse, monoclonal, Confirmed reactivity: Arabidopsis thaliana, Glycine ma 1,5 ml
AS05 093 Antibody: PrxQ | peroxiredoxin, thioredoxin reductase, Immunogen: His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column 200
AS10 1607 Antibody: CYP38 | cyclophilin 38, peptidyl-prolyl cis-trans isomerase (100 ul), Immunogen: recombinant full-length immunophilin (At3g01480 MIPS) from Arabidopsis thaliana, Host: rabbit, polyclonal, Co 100 ul
AS10 1618 Antibody: CYP38 | cyclophilin 38, peptidyl-prolyl cis-trans isomerase (300 ul), Immunogen: recombinant full-length immunophilin (At3g01480 MIPS) from Arabidopsis thaliana, Host: rabbit, polyclonal, Co 300 ul
AS07 219 Antibody: CCS | chloroplastic copper chaperone for SOD, Immunogen: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vect 200
AS08 326 Antibody: Sar1 | secretion-associated and Ras-related protein 1, Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7, Host: rabbit, polyclonal, Confirmed reactivity: A 200
AS09 531 Antibody: CBP80 | nuclear cap-binding protein subunit 1, Immunogen: recombinant, full length GST fusion of Arabidopsis thaliana CBP80 Q9SIU2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsi 200
AS07 258 Antibody: LOX-C | chloroplastic lipoxygenase, Immunogen: recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 476 Antibody: AtArf2 | ADP ribosylation factor 2, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 477 Antibody: S14 | 40S ribosomal protein S14-1, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1783 Antibody: Acd1 | accelerated cell death 1 , Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 515 Antibody: KC1 | potassium channel KAT3 , Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS09 474 Antibody: AtMTP4 | metal tolerance protein B, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 475 Antibody: Ligand-gated ion channel 1.3, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Raph 100
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
AS10 699 Antibody: AOX | alternative oxidase , Host: mouse, monoclonal, Comfirmed reactivity: Arabidopsis thaliana, Glycine max (AOX1,2,3), Sauromatum guttatum 1,5 ml
AS10 651 Antibody: Enolase, Immunogen: recombinant Arabidopsis thaliana enolase At2g36530, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 937 Antibody: CPLD11 , Immunogen: overexpressed fragment of recombinant Arabidopsis thaliana CPLD11 NP_566650, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 671 Antibody: AGO5 | argonaute 5, Immunogen: peptide of Arabidopsis thaliana AGO5 Q9SJK3, AT2G27880, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200


 

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