Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :19942850
Publication Date : //

Structural insights into the dual nucleotide exchange and GDI displacement activity of SidM/DrrA.


GDP-bound prenylated Rabs, sequestered by GDI (GDP dissociation inhibitor) in the cytosol, are delivered to destined sub-cellular compartment and subsequently activated by GEFs (guanine nucleotide exchange factors) catalysing GDP-to-GTP exchange. The dissociation of GDI from Rabs is believed to require a GDF (GDI displacement factor). Only two RabGDFs, human PRA-1 and Legionella pneumophila SidM/DrrA, have been identified so far and the molecular mechanism of GDF is elusive. Here, we present the structure of a SidM/DrrA fragment possessing dual GEF and GDF activity in complex with Rab1. SidM/DrrA reconfigures the Switch regions of the GTPase domain of Rab1, as eukaryotic GEFs do toward cognate Rabs. Structure-based mutational analyses show that the surface of SidM/DrrA, catalysing nucleotide exchange, is involved in GDI1 displacement from prenylated Rab1:GDP. In comparison with an eukaryotic GEF TRAPP I, this bacterial GEF/GDF exhibits high binding affinity for Rab1 with GDP retained at the active site, which appears as the key feature for the GDF activity of the protein.

Authors : Suh Hye-Young , Lee Dong-Won , Lee Kwang-Hoon , Ku Bonsu , Choi Sung-Jin , Woo Jae-Sung , Kim Yeon-Gil , Oh Byung-Ha ,

Related products :

Catalog number Product name Quantity
EIAAB35857 Kiaa0313,Mouse,Mus musculus,Neural RAP guanine nucleotide exchange protein,nRap GEP,PDZ domain-containing guanine nucleotide exchange factor 1,Pdzgef1,PDZ-GEF1,Rap guanine nucleotide exchange factor 2
EIAAB35856 Homo sapiens,Human,KIAA0313,Neural RAP guanine nucleotide exchange protein,nRap GEP,NRAPGEP,PDZ domain-containing guanine nucleotide exchange factor 1,PDZGEF1,PDZ-GEF1,RA-GEF,Rap guanine nucleotide ex
EIAAB35861 cAMP-dependent Rap1 guanine-nucleotide exchange factor,cAMP-GEFII,cAMP-regulated guanine nucleotide exchange factor II,Cgef2,EPAC 2,Epac2,Exchange factor directly activated by cAMP 2,Exchange protein
EIAAB39882 Deafness locus-associated putative guanine nucleotide exchange factor,DelGEF,DELGEF,GNEFR,Guanine nucleotide exchange factor-related protein,Homo sapiens,Human,Secretion-regulating guanine nucleotide
EIAAB39884 Deafness locus-associated putative guanine nucleotide exchange factor,DelGEF,Delgef,Gnefr,Guanine nucleotide exchange factor-related protein,Mouse,Mus musculus,Secretion-regulating guanine nucleotide
30-205 CCPG1 acts as an assembly platform for Rho protein signaling complexes. It limits guanine nucleotide exchange activity of MCF2L toward RHOA, which results in an inhibition of both its transcriptional 0.05 mg
EIAAB35866 GFR,Guanine nucleotide exchange factor for Rap1,Homo sapiens,Human,KIAA0277,M-Ras-regulated Rap GEF,MRGEF,MR-GEF,Rap guanine nucleotide exchange factor 5,RAPGEF5,Related to Epac,Repac
EIAAB34493 190 kDa guanine nucleotide exchange factor,Kiaa1998,Mouse,Mus musculus,p190RhoGEF,p190-RhoGEF,Rgnef,Rho-guanine nucleotide exchange factor,Rho-interacting protein 2,Rhoip2,Rip2
EIAAB34494 190 kDa guanine nucleotide exchange factor,Homo sapiens,Human,KIAA1998,p190RhoGEF,p190-RhoGEF,RGNEF,Rho-guanine nucleotide exchange factor
EIAAB35867 Homo sapiens,Human,PDZ domain-containing guanine nucleotide exchange factor 2,PDZGEF2,PDZ-GEF2,RA-GEF-2,Rap guanine nucleotide exchange factor 6,RAPGEF6
EIAAB35865 Gfr,Guanine nucleotide exchange factor for Rap1,Kiaa0277,Mouse,M-Ras-regulated Rap GEF,Mrgef,MR-GEF,Mus musculus,Rap guanine nucleotide exchange factor 5,Rapgef5
YSGKAMCC225E RanGEF (RCC), N_terminal, ~457kD, Clone 3D11, Mab anti_Human, Mouse, Rat_ WB_IP_ICC_NO inhibition of GEF (Guanine Nucleotide Exchange factor) activity of RanGEF 100 µg.
EIAAB34495 190 kDa guanine nucleotide exchange factor,p190RhoGEF,p190-RhoGEF,Rat,Rattus norvegicus,Rgnef,Rho-guanine nucleotide exchange factor
EIAAB35862 cAMP-GEFII,cAMP-regulated guanine nucleotide exchange factor II,Cgef2,EPAC 2,Epac2,Exchange factor directly activated by cAMP 2,Exchange protein directly activated by cAMP 2,Rap guanine nucleotide exc
EIAAB35869 Homo sapiens,Human,Link GEFII,Link guanine nucleotide exchange factor II,Rap guanine nucleotide exchange factor-like 1,RAPGEFL1
EIAAB27123 Ephexin-1,Eph-interacting exchange protein,Neuronal guanine nucleotide exchange factor,Ngef,Rat,Rattus norvegicus
EIAAB27125 Ephexin-1,Eph-interacting exchange protein,Mouse,Mus musculus,Neuronal guanine nucleotide exchange factor,Ngef
EIAAB27124 Ephexin-1,Eph-interacting exchange protein,Homo sapiens,Human,Neuronal guanine nucleotide exchange factor,NGEF
EIAAB35860 cAMP-GEFI,cAMP-regulated guanine nucleotide exchange factor I,Epac,EPAC 1,Epac1,Exchange factor directly activated by cAMP 1,Exchange protein directly activated by cAMP 1,Rap guanine nucleotide exchan
20-372-60216 deafness locus associated putative guanine nucleotide exchange factor (DELGEF) - Mouse monoclonal anti-human DELGEF antibody; Guanine nucleotide exchange factor-related protein; Deafness locus-associa 0.1 mg
EIAAB35863 cAMP-GEFII,cAMP-regulated guanine nucleotide exchange factor II,CGEF2,EPAC 2,EPAC2,Exchange factor directly activated by cAMP 2,Exchange protein directly activated by cAMP 2,Homo sapiens,Human,Rap gua
EIAAB35859 cAMP-GEFI,cAMP-regulated guanine nucleotide exchange factor I,CGEF1,EPAC,EPAC 1,EPAC1,Exchange factor directly activated by cAMP 1,Exchange protein directly activated by cAMP 1,Homo sapiens,Human,Rap
EIAAB35858 cAMP-GEFI,cAMP-regulated guanine nucleotide exchange factor I,Epac,EPAC 1,Epac1,Exchange factor directly activated by cAMP 1,Exchange protein directly activated by cAMP 1,Mouse,Mus musculus,Rap guanin
EIAAB35855 CRK SH3-binding GNRP,GRF2,Guanine nucleotide-releasing factor 2,Homo sapiens,Human,Protein C3G,Rap guanine nucleotide exchange factor 1,RAPGEF1
EIAAB34512 Kiaa0351,Mouse,Mus musculus,Ral GEF with PH domain and SH3-binding motif 1,Ral guanine nucleotide exchange factor 2,RalA exchange factor RalGPS1,RalGEF 2,Ralgef2,Ralgps1,Ralgps1a,Ras-specific guanine


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur