Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :20210363
Publication Date : //

Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8.


ABCG5 and ABCG8 are half-size ABC transporters that function as heterodimers (ABCG5/G8) to reduce sterol absorption in the intestines and increase sterol excretion from the liver. Previous studies demonstrated that bile acids increased ABCG5/G8 specific cholesterol efflux in cell models. In this study we tested the effects of bile acids on ATP hydrolysis in Pichia pastoris purified ABCG5/G8 and found that they stimulated hydrolysis approximately 20-fold in wild-type ABCG5/G8 but not in a hydrolysis-deficient mutant. Nonconjugated cholate supported the highest ATPase activity in ABCG5/G8 (256 +/- 9 nmol min(-1) mg(-1)). ATP hydrolysis was also stimulated by other conjugated bile acids and a mixture of bile acids resembling human bile with activities ranging from 129 +/- 4 to 147 +/- 14 nmol min(-1) mg(-1). The kinetic parameters, inhibitor profiles, and lipid requirements of bile acid stimulated ATP hydrolysis were characterized. Cholate-stimulated ATP hydrolysis was maximal at concentrations of >or=10 mM MgATP and had a relatively high K(M) (MgATP) of approximately 1 mM. Orthovanadate, BeFx, and AlFx effectively inhibited ABCG5/G8 at concentrations of 1 mM. Various lipid mixtures supported bile acid-stimulated ATP hydrolysis, which increased when cholesterol was present. The data demonstrate that bile acids together with lipids and cholesterol increase ATP hydrolysis in purified ABCG5/G8. Bile acids may promote an active conformation of purified ABCG5/G8 either by global stabilization of the transporter or by binding to a specific site on ABCG5/G8.

Authors : Johnson Brandy J Harvey , Lee Jyh-Yeuan , Pickert Amanda , Urbatsch Ina L ,

Related products :

Catalog number Product name Quantity
29-918 SLCO1A2 is a sodium-independent transporter which mediates cellular uptake of organic ions in the liver. Its substrates include bile acids, bromosulphophthalein, and some steroidal compounds. The prot 0.05 mg
18-461-10408 G-protein coupled bile acid receptor 1 - Membrane-type receptor for bile acids; M-BAR; hGPCR19; BG37; hBG37 Polyclonal 0.05 ml
18-461-10407 G-protein coupled bile acid receptor 1 - Membrane-type receptor for bile acids; M-BAR; hGPCR19; BG37; hBG37 Polyclonal 0.05 ml
18-461-10285 G-protein coupled bile acid receptor 1 - Membrane-type receptor for bile acids; M-BAR; hGPCR19; BG37; hBG37 Polyclonal 0.05 ml
18-461-10152 G-protein coupled bile acid receptor 1 - Membrane-type receptor for bile acids; M-BAR; hGPCR19; BG37; hBG37 Polyclonal 0.05 ml
26-066 BAAT is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The 0.05 mg
EIAAB06719 BAL,Bile salt-activated lipase,Bile salt-stimulated lipase,BSSL,Carboxyl ester lipase,Cel,Cholesterol esterase,Lip1,Mouse,Mus musculus,Pancreatic lysophospholipase,Sterol esterase
EIAAB06718 BAL,Bile salt-activated lipase,Bile salt-stimulated lipase,BSSL,Carboxyl ester lipase,Cel,Cholesterol esterase,Pancreatic lysophospholipase,Rat,Rattus norvegicus,Sterol esterase
EIAAB06720 BAL,Bile salt-activated lipase,Bile salt-stimulated lipase,Bos taurus,Bovine,BSSL,Carboxyl ester lipase,CEL,Cholesterol esterase,Pancreatic lysophospholipase,Sterol esterase
EIAAB06721 BAL,BAL,Bile salt-activated lipase,Bile salt-stimulated lipase,BSSL,Bucelipase,Carboxyl ester lipase,CEL,Cholesterol esterase,Homo sapiens,Human,Pancreatic lysophospholipase,Sterol esterase
EIAAB28024 Apical sodium-dependent bile acid transporter,ASBT,IBAT,Ileal Na(+)_bile acid cotransporter,Ileal sodium_bile acid cotransporter,Ileal sodium-dependent bile acid transporter,ISBT,Mouse,Mus musculus,Na
29-624 LCAT is an extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in its gene have been 0.1 mg
STREPIDENTIFICATIONKIT A panel of 12 tests for identification of Streptococcus species (Kit contains sterile broth for Esculin hydrolysis, Voges Proskauer test, Arginine utilization,PYR hydrolysis,ONPG Utilisation and 7 dif 1 kit (1 test)
EIAAB28027 Apical sodium-dependent bile acid transporter,ASBT,ASBT,Homo sapiens,Human,IBAT,Ileal Na(+)_bile acid cotransporter,Ileal sodium_bile acid cotransporter,Ileal sodium-dependent bile acid transporter,IS
EIAAB28026 Apical sodium-dependent bile acid transporter,ASBT,IBAT,Ileal Na(+)_bile acid cotransporter,Ileal sodium_bile acid cotransporter,Ileal sodium-dependent bile acid transporter,ISBT,Na(+)-dependent ileal
EIAAB28025 Apical sodium-dependent bile acid transporter,ASBT,IBAT,Ileal Na(+)_bile acid cotransporter,Ileal sodium_bile acid cotransporter,Ileal sodium-dependent bile acid transporter,ISBT,Na(+)-dependent ileal
2069-9954 NATIVE CANDIDA RUGOSA CHOLESTEROL ESTERASE, Product Type Purified Protein, Specificity CHOLESTEROL ESTERASE, Target Species Fungal, Host N_A, Format Purified, Isotypes , Applications E, FN, Clo 500 UNITS
SCH-2069-9954 NATIVE CANDIDA RUGOSA CHOLESTEROL ESTERASE, Product Type Purified Protein, Specificity CHOLESTEROL ESTERASE, Target Species Fungal, Host N_A, Format Purified, Isotypes , Applications E, FN, Clo 500 UNITS
551 BILE ACIDS, SERUM 1
BI2672 BILE ACIDS COLORIMETRIC 4th GEN. R1 10x10, R2 1x30
BI7982 BILE ACIDS COLORIMETRIC 5th GEN. R1 6x50, R2 6x18
BQ 092A-EALD Total Bile Acids (TBA) (Colorimetric) Kit 600/kit
BI3863 BILE ACIDS (Liquid) COLORIMETRIC 5th GEN. (RX daytona RX imola) R1 2x18, R2 2x8
26-776 KLB is a single-pass type III membrane protein. It contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. KLB is pr 0.05 mg
BQ 092A-EALD LIVER DISEASES-Total Bile Acids (TBA) (Colorimetric) Kit 600


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur