Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :23196131
Publication Date : //

Insights into subunit interactions within the insect olfactory receptor complex using FRET.


Insect olfactory receptors (ORs) are a novel family of ligand-gated cation channels that can respond to volatile organic compounds at low concentrations. They are involved in the detection of odorants associated with mate recognition, food localisation and predator avoidance. These receptors form a complex that is currently thought to contain at least two subunit members: the non-canonical Orco ion channel subunit and a ligand-binding receptor subunit. The integral membrane proteins SNMP1 and 2 are also associated with olfactory function, with SNMP1 required for cis-vaccinyl acetate reception in Drosophila melanogaster. In order to investigate protein-protein interactions among these membrane proteins we measured intermolecular Förster/Fluorescence Resonance Energy Transfer (FRET) in live insect cells by acceptor photobleaching. Fusion proteins containing Cyan Fluorescent Protein or Yellow Fluorescent Protein were produced using baculovirus-mediated expression in High Five™ cells. The majority of the recombinant products were of the expected size for the fusion proteins and located within intracellular membranes. We were able to show FRET efficiencies providing evidence for homomeric and heteromeric interactions of the ligand-binding OR, Or22a, and Orco (Or22a-Or22a, Or22a-Orco, Orco-Orco). There was no evidence for an interaction between SNMP1 and Orco or between SNMP2 and Orco or Or22a. However, fusion proteins of SNMP1 and Or22a did show an interaction by FRET, suggesting SNMP1 may interact with at least some insect olfactory receptor complexes. In summary, this study supports previously observed homomeric and heteromeric interactions between Orco and the ligand-binding OR, Or22a, and identifies a novel interaction between Or22a and SNMP1.

Authors : German Pablo F , van der Poel Selene , Carraher Colm , Kralicek Andrew V , Newcomb Richard D ,

Related products :

Catalog number Product name Quantity
EIAAB28960 Homo sapiens,Human,Olfactory receptor 1Q1,Olfactory receptor 1Q2,Olfactory receptor 1Q3,Olfactory receptor 9-A,Olfactory receptor OR9-25,Olfactory receptor TPCR106,OR1Q1,OR1Q2,OR1Q3,OR9-A,OST226
EIAAB28939 Homo sapiens,Human,Olfactory receptor 13-66,Olfactory receptor 17-2_17-32,Olfactory receptor 1E1,Olfactory receptor 1E5,Olfactory receptor 1E6,Olfactory receptor 5-85,Olfactory receptor OR17-18,Olfact
EIAAB28942 Homo sapiens,Human,Olfactory receptor 16-35,Olfactory receptor 1F1,Olfactory receptor 1F10,Olfactory receptor 1F4,Olfactory receptor 1F5,Olfactory receptor 1F6,Olfactory receptor 1F7,Olfactory recepto
EIAAB28982 Homo sapiens,Hs6M1-16,Human,Olfactory receptor 2H1,Olfactory receptor 2H6,Olfactory receptor 2H8,Olfactory receptor 6-2,Olfactory receptor OR6-32,OLFR42A-9004.14_9026.2,OR2H1,OR2H6,OR2H8,OR6-2
EIAAB29018 Homo sapiens,Human,Olfactory receptor 17-201,Olfactory receptor 3A3,Olfactory receptor 3A6,Olfactory receptor 3A7,Olfactory receptor 3A8,Olfactory receptor OR17-22,OR17-201,OR3A3,OR3A6,OR3A7,OR3A8P
EIAAB28970 Homo sapiens,Hs6M1-32,Human,Olfactory receptor 2B1,Olfactory receptor 2B5,Olfactory receptor 2B6,Olfactory receptor 5-40,Olfactory receptor 6-31,Olfactory receptor OR6-4,OR2B1,OR2B1P,OR2B5,OR2B6,OR2B6
EIAAB28977 Homo sapiens,Human,OLF3,Olfactory receptor 2F1,Olfactory receptor 2F3,Olfactory receptor 2F4,Olfactory receptor 2F5,Olfactory receptor-like protein OLF3,OR2F1,OR2F3,OR2F3P,OR2F4,OR2F5
EIAAB28789 Olfactory receptor 1,Olfactory receptor 1469,Olfactory receptor HGL-SP1,Olfactory receptor OR5,Olfactory receptor-like protein I54,Olfr1,Olr1469,Rat,Rattus norvegicus
EIAAB28409 Homo sapiens,Human,Olfactory receptor 10P1,Olfactory receptor 10P2,Olfactory receptor 10P3,Olfactory receptor OR12-7,OR10P1,OR10P1P,OR10P2P,OR10P3P
EIAAB28483 Homo sapiens,Human,Olfactory receptor 4F11,Olfactory receptor 4F17,Olfactory receptor 4F18,Olfactory receptor 4F19,OR4F11P,OR4F17,OR4F18,OR4F19
EIAAB28381 Homo sapiens,HP3,Human,Olfactory receptor 10A1,Olfactory receptor 10A5,Olfactory receptor 11-403,Olfactory receptor-like protein JCG6,OR10A1,OR10A5,OR11-403
EIAAB28948 Homo sapiens,HSA5,HTPCRX15,Human,Olfactory receptor 1J2,Olfactory receptor 1J3,Olfactory receptor 1J5,Olfactory receptor OR9-19,OR1J2,OR1J3,OR1J5,OST044
EIAAB29146 Homo sapiens,Human,Olfactory receptor 8D1,Olfactory receptor 8D3,Olfactory receptor OR11-301,Olfactory receptor-like protein JCG9,OR8D1,OR8D3,OST004
EIAAB29150 Homo sapiens,Human,Olfactory receptor 8G2,Olfactory receptor 8G4,Olfactory receptor OR11-297,Olfactory receptor TPCR120,OR8G2,OR8G2P,OR8G4
EIAAB28966 Homo sapiens,Human,Olfactory receptor 2A26,Olfactory receptor 2A5,Olfactory receptor 2A8,Olfactory receptor 7-138_7-141,OR2A26,OR2A5,OR2A8,OR7-138,OR7-141
EIAAB29105 Homo sapiens,hP2 olfactory receptor,Human,Olfactory receptor 11-55,Olfactory receptor 6A1,Olfactory receptor 6A2,Olfactory receptor OR11-83,OR11-55,OR6A1,OR6A2,OR6A2P
EIAAB29133 Homo sapiens,Human,Olfactory receptor 7C1,Olfactory receptor 7C4,Olfactory receptor OR19-16,Olfactory receptor TPCR86,OR7C1,OR7C4
EIAAB28974 Homo sapiens,Human,Olfactory receptor 2C3,Olfactory receptor 2C4,Olfactory receptor 2C5,Olfactory receptor OR1-30,OR2C3,OR2C4,OR2C5P
EIAAB28953 Homo sapiens,Human,Olfactory receptor 1L4,Olfactory receptor 1L5,Olfactory receptor 9-E,Olfactory receptor OR9-29,OR1L4,OR1L5,OR9-E,OST046
EIAAB08492 COG complex subunit 1,Cog1,Component of oligomeric Golgi complex 1,Conserved oligomeric Golgi complex subunit 1,Ldlb,Low density lipoprotein receptor defect B-complementing protein,Mouse,Mus musculus
EIAAB08495 COG complex subunit 2,Cog2,Component of oligomeric Golgi complex 2,Conserved oligomeric Golgi complex subunit 2,Ldlc,Low density lipoprotein receptor defect C-complementing protein,Mouse,Mus musculus
EIAAB08494 COG complex subunit 2,COG2,Component of oligomeric Golgi complex 2,Conserved oligomeric Golgi complex subunit 2,Homo sapiens,Human,LDLC,Low density lipoprotein receptor defect C-complementing protein
EIAAB33312 Complex III subunit 8,Complex III subunit VIII,Cytochrome b-c1 complex subunit 8,Low molecular mass ubiquinone-binding protein,Qpc,Rat,Rattus norvegicus,Ubiquinol-cytochrome c reductase complex 9.5 kD
EIAAB33299 Complex III subunit 10,Complex III subunit XI,Cytochrome b-c1 complex subunit 10,Homo sapiens,Human,Ubiquinol-cytochrome c reductase complex 6.4 kDa protein,UQCR,UQCR11
EIAAB33310 Complex III subunit 7,Complex III subunit VII,Cytochrome b-c1 complex subunit 7,Homo sapiens,Human,QP-C,Ubiquinol-cytochrome c reductase complex 14 kDa protein,UQBP,UQCRB


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur