Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :23275093
Publication Date : //

Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms.


Avian pathogenic Escherichia coli (APEC) causes respiratory and systemic disease in poultry. Sequencing of a multilocus sequence type 95 (ST95) serogroup O1 strain previously indicated that APEC resembles E. coli causing extraintestinal human diseases. We sequenced the genomes of two strains of another dominant APEC lineage (ST23 serogroup O78 strains χ7122 and IMT2125) and compared them to each other and to the reannotated APEC O1 sequence. For comparison, we also sequenced a human enterotoxigenic E. coli (ETEC) strain of the same ST23 serogroup O78 lineage. Phylogenetic analysis indicated that the APEC O78 strains were more closely related to human ST23 ETEC than to APEC O1, indicating that separation of pathotypes on the basis of their extraintestinal or diarrheagenic nature is not supported by their phylogeny. The accessory genome of APEC ST23 strains exhibited limited conservation of APEC O1 genomic islands and a distinct repertoire of virulence-associated loci. In light of this diversity, we surveyed the phenotype of 2,185 signature-tagged transposon mutants of χ7122 following intra-air sac inoculation of turkeys. This procedure identified novel APEC ST23 genes that play strain- and tissue-specific roles during infection. For example, genes mediating group 4 capsule synthesis were required for the virulence of χ7122 and were conserved in IMT2125 but absent from APEC O1. Our data reveal the genetic diversity of E. coli strains adapted to cause the same avian disease and indicate that the core genome of the ST23 lineage serves as a chassis for the evolution of E. coli strains adapted to cause avian or human disease via acquisition of distinct virulence genes.

Authors : Dziva Francis , Hauser Heidi , Connor Thomas R , van Diemen Pauline M , Prescott Graham , Langridge Gemma C , Eckert Sabine , Chaudhuri Roy R , Ewers Christa , Mellata Melha , Mukhopadhyay Suman , Curtiss Roy , Dougan Gordon , Wieler Lothar H , Thomson Nicholas R , Pickard Derek J , Stevens Mark P ,

Related products :

Catalog number Product name Quantity
IP21001 Avian pathogenic Escherichia coli (APEC) 48T
B475 Crystal Violet Lactose Agar USE : For differentiation of pure cultures of pathogenic and non pathogenic Staphylococci. 5x500gm
B475 Crystal Violet Lactose Agar USE For differentiation of pure cultures of pathogenic and non pathogenic Staphylococci. Qty per Litre of Medium: 33
B009 YT Broth USE For the cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 31
B009 YT Broth USE : For the cultivation of recombinant strains of Escherichia coli. 5x500gm
TM 696 CASEIN YEAST MAGNESIUM AGAR (for cultivation of recombinant strains of Escherichia coli.) 500 gm
B970 Casein Magnesium Broth USE For use in the cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 16
B970 Casein Magnesium Broth USE : For use in the cultivation of recombinant strains of Escherichia coli. 5x500gm
B1057 Hanahan’s Broth (SOB Medium) USE For use in cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 28
B1057 Hanahan’s Broth (SOB Medium) USE : For use in cultivation of recombinant strains of Escherichia coli. 5x500gm
B971 Casein Yeast Magnesium Agar USE : For cultivation of recombinant strains of Escherichia coli. 5x500gm
B972 Casein Yeast Magnesium Broth USE : For cultivation of recombinant strains of Escherichia coli. 5x500gm
B972 Casein Yeast Magnesium Broth USE For cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 21
B971 Casein Yeast Magnesium Agar USE For cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 35,98
B753 MacConkey Sorbitol Agar USE For isolation and identification of enteropathogenic Escherichia coli strains associated with infant diarrhoea. Qty per Litre of Medium: 50
B753 MacConkey Sorbitol Agar USE : For isolation and identification of enteropathogenic Escherichia coli strains associated with infant diarrhoea. 5x500gm
B087 Tartoff_Hobbs Broth (Terrific Broth) USE For the cultivation of recombinant strains of Escherichia coli. Qty per Litre of Medium: 47,6
B087 Tartoff-Hobbs Broth (Terrific Broth) USE : For the cultivation of recombinant strains of Escherichia coli. 5x500gm
B699 Miller Luria Bertani Broth USE For the cultivation and maintenance of recombinant strains of Escherichia coli and can be used for routine cultivation of not particularly fastidious microorganisms. Qty per Litre of Medium: 25
B699 Miller Luria Bertani Broth USE : For the cultivation and maintenance of recombinant strains of Escherichia coli and can be used for routine cultivation of not particularly fastidious microorganisms. 5x500gm
B699 Luria Bertani Broth,Miller USE For the cultivation and maintenance of recombinant strains of Escherichia coli and can be used for routine cultivation of not particularly fastidious microorganisms Qty per Litre of Medium: 25
B699 Luria Bertani Broth,Miller USE For the cultivation and maintenance of recombinant strains of Escherichia coli and can be used for routine cultivation of not particularly fastidious microorganisms Qty per Litre of Medium: 25
B699 Luria Bertani Broth,Miller USE : For the cultivation and maintenance of recombinant strains of Escherichia coli and can be used for routine cultivation of not particularly fastidious microorganisms 5x500gm
B700 Miller Luria Bertani Agar USE For the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies,can be used for routine cultivation and isolation of no Qty per Litre of Medium: 40
B700 Luria Bertani Agar,Miller USE For the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies,can be used for routine cultivation and isolation of Qty per Litre of Medium: 40


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur