Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :23826237
Publication Date : //

Identification of a Novel TGF-β-Binding Site in the Zona Pellucida C-terminal (ZP-C) Domain of TGF-β-Receptor-3 (TGFR-3).


The zona pellucida (ZP) domain is present in extracellular proteins such as the zona pellucida proteins and tectorins and participates in the formation of polymeric protein networks. However, the ZP domain also occurs in the cytokine signaling co-receptor transforming growth factor β (TGF-β) receptor type 3 (TGFR-3, also known as betaglycan) where it contributes to cytokine ligand recognition. Currently it is unclear how the ZP domain architecture enables this dual functionality. Here, we identify a novel major TGF-β-binding site in the FG loop of the C-terminal subdomain of the murine TGFR-3 ZP domain (ZP-C) using protein crystallography, limited proteolysis experiments, surface plasmon resonance measurements and synthetic peptides. In the murine 2.7 Å crystal structure that we are presenting here, the FG-loop is disordered, however, well-ordered in a recently reported homologous rat ZP-C structure. Surprisingly, the adjacent external hydrophobic patch (EHP) segment is registered differently in the rat and murine structures suggesting that this segment only loosely associates with the remaining ZP-C fold. Such a flexible and temporarily-modulated association of the EHP segment with the ZP domain has been proposed to control the polymerization of ZP domain-containing proteins. Our findings suggest that this flexibility also extends to the ZP domain of TGFR-3 and might facilitate co-receptor ligand interaction and presentation via the adjacent FG-loop. This hints that a similar C-terminal region of the ZP domain architecture possibly regulates both the polymerization of extracellular matrix proteins and cytokine ligand recognition of TGFR-3.

Authors : Diestel Uschi , Resch Marcus , Meinhardt Kathrin , Weiler Sigrid , Hellmann Tina V , Mueller Thomas D , Nickel Joachim , Eichler Jutta , Muller Yves A ,

Related products :

Catalog number Product name Quantity
EIAAB48011 Homo sapiens,Human,Sperm receptor,Zona pellucida glycoprotein 3,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,ZP3A,ZP3A_ZP3B,ZP3B,ZPC
EIAAB48010 Bos taurus,Bovine,Sperm receptor,Zona pellucida glycoprotein 3,Zona pellucida glycoprotein 3B,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,Zp-3B,ZPC
EIAAB48015 Oryctolagus cuniculus,Rabbit,Sperm receptor,Zona pellucida glycoprotein 3,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,ZPC
EIAAB48002 75 kDa zona pellucida protein,Oryctolagus cuniculus,Rabbit,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,ZP2,Zp-2,ZPA
EIAAB48019 Oryctolagus cuniculus,Rabbit,RC55,Zona pellucida glycoprotein 4,Zona pellucida glycoprotein X,Zona pellucida protein B,Zona pellucida sperm-binding protein 4,ZP4,Zp-4,ZPB,ZPX,Zp-X
EIAAB48012 Mouse,Mus musculus,Sperm receptor,Zona pellucida glycoprotein 3,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,Zp3,Zp-3,Zp-3,Zpc
18-003-42708 Zona pellucida sperm-binding protein 3 - Zona pellucida glycoprotein ZP3; Zona pellucida protein C; Sperm receptor; ZP3A_ZP3B Polyclonal 0.1 mg Protein A
EIAAB48009 Pig,Sperm receptor,Sus scrofa,Zona pellucida glycoprotein 3,Zona pellucida glycoprotein 3-beta,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,ZP3B,Zp-3-beta,Zp3-beta,ZPC
EIAAB48003 Homo sapiens,Human,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,ZP2,Zp-2,ZPA
EIAAB48020 Homo sapiens,Human,Zona pellucida glycoprotein 4,Zona pellucida protein B,Zona pellucida sperm-binding protein 4,ZP4,Zp-4,ZPB
EIAAB48004 Bos taurus,Bovine,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,ZP2,Zp-2,ZPA
EIAAB48006 Rat,Rattus norvegicus,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,Zp2,Zp-2,Zpa
EIAAB48013 Rat,Rattus norvegicus,Zona pellucida glycoprotein 3,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,Zp3,Zp-3,Zp-3,Zpc
EIAAB48021 Rat,Rattus norvegicus,Zona pellucida glycoprotein 4,Zona pellucida protein B,Zona pellucida sperm-binding protein 4,Zp4,Zp-4,Zpb
EIAAB48022 Bos taurus,Bovine,Zona pellucida glycoprotein 4,Zona pellucida protein B,Zona pellucida sperm-binding protein 4,ZP4,Zp-4,ZPB
EIAAB48005 Mouse,Mus musculus,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,Zp2,Zp-2,Zp-2,Zpa
EIAAB48014 Chicken,Gallus gallus,Sperm receptor,Zona pellucida C protein,Zona pellucida glycoprotein 3,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,ZPC
EIAAB48001 Pig,Sus scrofa,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,ZP1,ZP2,Zp-2,ZPA
EIAAB48008 Canis familiaris,Canis lupus familiaris,Dog,Sperm receptor,Zona pellucida glycoprotein 3,Zona pellucida protein C,Zona pellucida sperm-binding protein 3,ZP3,Zp-3,ZPC
EIAAB48018 Pig,Sus scrofa,Zona pellucida glycoprotein 3-alpha,Zona pellucida glycoprotein 4,Zona pellucida protein B,Zona pellucida sperm-binding protein 4,ZP3A,Zp-3-alpha,Zp3-alpha,ZP4,Zp-4,ZPB
EIAAB48000 Homo sapiens,Human,Zona pellucida glycoprotein 1,Zona pellucida sperm-binding protein 1,ZP1,Zp-1
EIAAB47999 Rat,Rattus norvegicus,Zona pellucida glycoprotein 1,Zona pellucida sperm-binding protein 1,Zp1,Zp-1
EIAAB47998 Mouse,Mus musculus,Zona pellucida glycoprotein 1,Zona pellucida sperm-binding protein 1,Zp1,Zp-1
EIAAB48007 Canis familiaris,Canis lupus familiaris,Dog,Zona pellucida glycoprotein 2,Zona pellucida protein A,Zona pellucida sperm-binding protein 2,ZP2,Zp-2,ZPA
ZP3R_MOUSE Mouse ELISA Kit FOR Zona pellucida sperm-binding protein 3 receptor 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur