Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :24223817
Publication Date : //

Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows.


The ruminal microbial community is a unique source of enzymes that underpin the conversion of cellulosic biomass. In this study, the microbial consortia adherent on solid digesta in the rumen of Jersey cattle were subjected to an activity-based metagenomic study to explore the genetic diversity of carbohydrolytic enzymes in Jersey cows, with a particular focus on cellulases and xylanases. Pyrosequencing and bioinformatic analyses of 120 carbohydrate-active fosmids identified genes encoding 575 putative Carbohydrate-Active Enzymes (CAZymes) and proteins putatively related to transcriptional regulation, transporters, and signal transduction coupled with polysaccharide degradation and metabolism. Most of these genes shared little similarity to sequences archived in databases. Genes that were predicted to encode glycoside hydrolases (GH) involved in xylan and cellulose hydrolysis (e.g., GH3, 5, 9, 10, 39 and 43) were well represented. A new subfamily (S-8) of GH5 was identified from contigs assigned to Firmicutes. These subfamilies of GH5 proteins also showed significant phylum-dependent distribution. A number of polysaccharide utilization loci (PULs) were found, and two of them contained genes encoding Sus-like proteins and cellulases that have not been reported in previous metagenomic studies of samples from the rumens of cows or other herbivores. Comparison with the large metagenomic datasets previously reported of other ruminant species (or cattle breeds) and wallabies showed that the rumen microbiome of Jersey cows might contain differing CAZymes. Future studies are needed to further explore how host genetics and diets affect the diversity and distribution of CAZymes and utilization of plant cell wall materials.

Authors : Wang Lingling , Hatem Ayat , Catalyurek Umit V , Morrison Mark , Yu Zhongtang ,

Related products :

Catalog number Product name Quantity
B731 Soyabean Casein Digest Medium(w_o Dextrose) USE : For cultivation of anaerobic microorganisms when the presence of carbohydrate is not desired. 5x500gm
B731 Soyabean Casein Digest Medium(w_o Dextrose) USE For cultivation of anaerobic microorganisms when the presence of carbohydrate is not desired. Qty per Litre of Medium: 27,5
B407 Purple Broth Base USE For preparation of carbohydrate media used in fermentation studies for the cultural identification of pure cultures of enteric and other microorganisms. Qty per Litre of Medium: 15
B696 Purple Agar Base USE : For preparation of carbohydrate media used in fermentation studies for the cultural identification of pure cultures of enteric and other microorganisms. 5x500gm
B407 Purple Broth Base USE : For preparation of carbohydrate media used in fermentation studies for the cultural identification of pure cultures of enteric and other microorganisms. 5x500gm
B696 Purple Agar Base USE For preparation of carbohydrate media used in fermentation studies for the cultural identification of pure cultures of enteric and other microorganisms. Qty per Litre of Medium: 31
08108 Adhering Mat, Blue, 18" x 18" 1
08108 Adhering Mat, Blue, 18" x 18"
32-159 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversib 0.1 mL
0871 Orbital_Genie Shaker, Adhering Mat, 120V
0871 Orbital_Genie Shaker, Adhering Mat, 120V 1
25-894 Zinc is an essential cofactor for more than 50 classes of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, 0.05 mg
0874 Orbital_Genie Shaker, Adhering Mat, 230V, No Plug 1
0874 Orbital_Genie Shaker, Adhering Mat, 230V, No Plug
25-932 Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development 0.05 mg
29-956 Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development 0.05 mg
29-923 Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development 0.1 mg
orb61256 Lapatinib (free base) Lapatinib, used in the form of lapatinib ditosylate, (USAN) (Tykerb_Tyverb, GSK) is an orally active drug for breast cancer and other solid tumours. For research use only. 100 mg
26-373 B3GALNT2 is the beta-1,3-N-acetylgalactosaminyltransferase active in synthesizing a unique carbohydrate structure, GalNAc-beta-1-3GlcNAc, on N- and O-glycans. B3GALNT2 has no galactose nor galactosami 0.05 mg
BCR100/03 Carbohydrate Miniposter "Cell Surface Carbohydrate Interactions" unit
B005 Yeast Nitrogen Base Agar (Twin Pack) USE : For assessing carbohydrate utilizing ability of yeasts using carbohydrate disc method. 5x500gm
B005 *Yeast Nitrogen Base Agar (Twin Pack) USE For assessing carbohydrate utilizing ability of yeasts using carbohydrate disc method. Qty per Litre of Medium: 80.00 of part A + 13.00 of part B
orb61257 Lapatinib (ditosylate salt) Lapatinib, used in the form of lapatinib ditosylate, (USAN) (Tykerb_Tyverb, GSK) is an orally active drug for breast cancer and other solid tumours. For research use only. 100 mg
30-517 The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is 0.1 mg
30-134 The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur