Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :24275569
Publication Date : 2014/01/27

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.


Protein phosphorylation is one of the most common post-translational modifications. It plays key roles in regulating diverse biological processes of liver tissues. To better understand the role of protein phosphorylation in liver functions, it is essential to perform in-depth phosphoproteome analysis of human liver. Here, an enzyme assisted reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach with both RPLC separations operated with optimized acidic mobile phase was developed. High orthogonal separation was achieved by trypsin digestion of the Glu-C generated peptides in the fractions collected from the first RPLC separation. The phosphoproteome coverage was further improved by using two types of instruments, i.e. TripleTOF 5600 and LTQ Orbitrap Velos. A total of 22,446 phosphorylation sites, corresponding to 6526 nonredundant phosphoproteins were finally identified from normal human liver tissues. Of these sites, 15,229 sites were confidently localized with Ascore≥13. This dataset was the largest phosphoproteome dataset of human liver. It can be a public resource for the liver research community and holds promise for further biology studies.

Authors : Bian Yangyang , Song Chunxia , Cheng Kai , Dong Mingming , Wang Fangjun , Huang Junfeng , Sun Deguang , Wang Liming , Ye Mingliang , Zou Hanfa ,

Related products :

Catalog number Product name Quantity
28-376 Matrix-assisted laser desorption ionization-time of flight analysis and examination of protein profiles from the SwissProt database revealed that the previously defined p97 repressor is ZNF224, a zinc 0.1 mg
XGC-BiRDS-WGS-Hyb-Asmbl3 Bioinformatics of MiSeq and NextSeq 500 data using Hybrid approach for Denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl1 Bioinformatics of Roche and MiSeq data using hybrid approach for denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl2 Bioinformatics of Roche, MiSeq and NextSeq 500 data using Hybrid approach for Denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl5 Bioinformatics of MiSeq and NextSeq 500 per HiSeq 2000 data using Hybrid approach for Denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl4 Bioinformatics of Roche, MiSeq and NextSeq 500 per HiSeq 2000 data using Hybrid approach for Denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl6 Bioinformatics of MiSeq, NextSeq 500 per HiSeq 2000 and PacBio data using Hybrid approach for Denovo genome analysis Sample
XGC-BiRDS-WGS-Hyb-Asmbl7 Bioinformatics of Roche, MiSeq, NextSeq 500 per HiSeq 2000 and Pacbio data using Hybrid approach for Denovo genome analysis Sample
1239 LIVER HYDROLYSATE POWDER (HYDROLYSIS OF LIVER BY ENZYME) (ingredient of culture media used for cultivation of anaerobes) 500 gm
E0703h ELISA kit ACR1,Alu corepressor 1,Antioxidant enzyme B166,AOEB166,Homo sapiens,Human,Liver tissue 2D-page spot 71B,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,PRD 96T
SB0084 Restriction enzyme (Please send us the name of the Restriction enzyme, we will send you quotation) ,CHEMICALS AND CONSUMERABLES FOR PCR ANALYSIS, 1000 IU
E0703h ELISA ACR1,Alu corepressor 1,Antioxidant enzyme B166,AOEB166,Homo sapiens,Human,Liver tissue 2D-page spot 71B,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,PRDX5,Pr 96T
U0703h CLIA ACR1,Alu corepressor 1,Antioxidant enzyme B166,AOEB166,Homo sapiens,Human,Liver tissue 2D-page spot 71B,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,PRDX5,Prx 96T
KMCH35 Enema And Assisted Defecation Training Simulator
KMC15 Enema and Assisted Defecation Training Model
E0703m ELISA kit Antioxidant enzyme B166,AOEB166,Liver tissue 2D-page spot 2D-0014IV,Mouse,Mus musculus,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,Prdx5,Prdx6,Prx-V,Th 96T
U0703m CLIA Antioxidant enzyme B166,AOEB166,Liver tissue 2D-page spot 2D-0014IV,Mouse,Mus musculus,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,Prdx5,Prdx6,Prx-V,Thioredo 96T
E0703m ELISA Antioxidant enzyme B166,AOEB166,Liver tissue 2D-page spot 2D-0014IV,Mouse,Mus musculus,Peroxiredoxin V,Peroxiredoxin-5, mitochondrial,Peroxisomal antioxidant enzyme,PLP,Prdx5,Prdx6,Prx-V,Thiored 96T
TotalLabQuant Base package*: 1D image analysis plus colony counting and array analysis
AS-MRA-P5 MARIA™ ANALYSIS SERVICE 5-plex analysis Environmental Testing Services 1 sample
AS-MRA-P8 MARIA™ ANALYSIS SERVICE 8-plex analysis Environmental Testing Services 1 sample
XGC-BiRDS-MeDIP-Seq Bioinformatics analysis of Methylome data for Methylation analysis Sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis 1
XGC-BiRDS-WGBS Bioinformatics analysis of Whole Genome Bisulphite Sequencing data for Methylation analysis Sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur