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Pubmed ID :2581967
Publication Date : //

Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor.


Ubiquitin is a 76-amino acid protein whose sequence is highly conserved throughout evolution from invertebrates to mammals. It is both a cytoplasmic and nuclear protein. In the cytoplasm it is involved in ATP-dependent nonlysosomal proteolysis. In the nucleus, ubiquitin is conjugated to histone 2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. During attempts to identify a cDNA encoding somatomedin-C (insulin-like growth factor I) we screened a fetal human liver cDNA library with a mixture of 17 base oligonucleotides corresponding to a portion of the B chain of somatomedin-C. One oligonucleotide of the mixture hybridized to two cDNAs encoding ubiquitin despite a 2-base pair mismatch. Nucleotide sequence analyses of the 350- and 516-base pair cDNAs revealed that they correspond to the same ubiquitin mRNA. The coding sequence of the 516-base pair cDNA begins at amino acid 5 of the ubiquitin sequence and encodes amino acids 5 through 76 of ubiquitin, an 80-amino acid carboxy-terminal extension, a 3' untranslated region, and a poly(A) tail. The finding that ubiquitin is synthesized as a precursor raises the possibility that the precursor sequence may be important in compartmentalization of ubiquitin or ubiquitin precursors. Analyses of ubiquitin mRNAs in poly(A) RNA extracted from human liver and various rat tissues reveals that there are three distinct mRNAs encoding ubiquitin in humans and four mRNAs in the rat.

Authors : Lund P K , Moats-Staats B M , Simmons J G , Hoyt E , D'Ercole A J , Martin F , Van Wyk J J ,

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EIAAB45086 Deubiquitinating enzyme 51,Homo sapiens,Human,Ubiquitin carboxyl-terminal hydrolase 51,Ubiquitin thiolesterase 51,Ubiquitin-specific-processing protease 51,USP51
EIAAB45029 Deubiquitinating enzyme 29,Homo sapiens,Human,Ubiquitin carboxyl-terminal hydrolase 29,Ubiquitin thiolesterase 29,Ubiquitin-specific-processing protease 29,USP29
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EIAAB44995 Deubiquitinating enzyme 17,Homo sapiens,Human,Ubiquitin carboxyl-terminal hydrolase 17,Ubiquitin thiolesterase 17,Ubiquitin-specific-processing protease 17,USP17
EIAAB45080 Deubiquitinating enzyme 49,Homo sapiens,Human,Ubiquitin carboxyl-terminal hydrolase 49,Ubiquitin thiolesterase 49,Ubiquitin-specific-processing protease 49,USP49
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