Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :3443302
Publication Date : //

Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases.


There are at least three alkaline phosphatase (AP) isoenzymes in man: a heat-stable placental enzyme (PLAP), a less heat-stable intestinal form (IAP), and the very heat-labile AP enriched in liver, bone and kidney. In addition to these enzymes, there is a heat-stable activity in the thymus and testis that is similar but not identical to the PLAP (the PLAP-like enzyme). Previous work has demonstrated a close structural relatedness among the IAP, PLAP and PLAP-like enzymes. Thus, it is possible that there are three human genes encoding heat-stable AP enzymes. To test this hypothesis, we have used a PLAP cDNA clone to screen a human genomic library cloned into the phage vector lambda EMBL-3. Three sets of clones were isolated, each bearing a distinct coding region homologous to the PLAP cDNA probe. Nucleotide sequence analysis of the 5' ends of these genes allowed comparison of their derived peptide sequences and positive identification of two of the genes. One of the genes encodes the PLAP (the PLAP-1 gene), another encodes the IAP, and a third closely resembles the PLAP-1 gene, but is distinct from it (the PLAP-2 gene). The PLAP-2 gene is highly homologous (greater than 95%) with the PLAP-1 except in the first exon, where sequences encoding the hydrophobic signal peptide are nearly identical with the same region of the IAP gene. These results demonstrate the existence of a small family of PLAP-related genes which is the result of at least two duplication events during the descent of man.

Authors : Knoll B J , Rothblum K N , Longley M ,

Related products :

Catalog number Product name Quantity
orb60758 Sodium orthovanadate Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43. It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of 10 mg
29-787 There are at least four distinct but related alkaline phosphatases intestinal, placental, placental-like, and liver_bone_kidney (tissue non-specific). The exact physiological function of the alkaline 0.1 mg
CHSP1_HUMAN ELISA Kit FOR Calcium-regulated heat stable protein 1; organism: Human; gene name: CARHSP1 96T
EIAAB07260 Calcium-regulated heat stable protein 1,Calcium-regulated heat-stable protein of 24 kDa,CARHSP1,CRHSP-24,Homo sapiens,Human
E2189m ELISA kit Cd24,Cd24a,HSA,Ly-52,Ly-52,Lymphocyte antigen 52,M1_69-J11D heat stable antigen,Mouse,Mus musculus,Nectadrin,R13-Ag,Signal transducer CD24,X62 heat stable antigen 96T
U2189m CLIA Cd24,Cd24a,HSA,Ly-52,Ly-52,Lymphocyte antigen 52,M1_69-J11D heat stable antigen,Mouse,Mus musculus,Nectadrin,R13-Ag,Signal transducer CD24,X62 heat stable antigen 96T
E2189m ELISA Cd24,Cd24a,HSA,Ly-52,Ly-52,Lymphocyte antigen 52,M1_69-J11D heat stable antigen,Mouse,Mus musculus,Nectadrin,R13-Ag,Signal transducer CD24,X62 heat stable antigen 96T
U2189m CLIA kit Cd24,Cd24a,HSA,Ly-52,Ly-52,Lymphocyte antigen 52,M1_69-J11D heat stable antigen,Mouse,Mus musculus,Nectadrin,R13-Ag,Signal transducer CD24,X62 heat stable antigen 96T
M1034 Fluorescein diphosphate, tetraammonium salt, Fluorescent Substrate for Alkaline Phosphatases, 5mg
E2189m Cd24,Cd24a,HSA,Ly-52,Ly-52,Lymphocyte antigen 52,M1_69-J11D heat stable antigen,Mouse,Mus musculus,Nectadrin,R13-Ag,Signal transducer CD24,X62 heat stable antigen
29-235 This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of t 0.05 mg
29-509 This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of t 0.05 mg
CARM1 CARHSP1 Gene calcium regulated heat stable protein 1, 24kDa
GUCY2F GUCY2C Gene guanylate cyclase 2C (heat stable enterotoxin receptor)
GUC2C_MOUSE ELISA Kit FOR Heat-stable enterotoxin receptor; organism: Mouse; gene name: Gucy2c 96T
CHSP1_MOUSE ELISA Kit FOR Calcium-regulated heat stable protein 1; organism: Mouse; gene name: Carhsp1 96T
EIAAB07259 Calcium-regulated heat stable protein 1,Calcium-regulated heat-stable protein of 24 kDa,Carhsp1,CRHSP-24,Rat,Rattus norvegicus
EIAAB07261 Calcium-regulated heat stable protein 1,Calcium-regulated heat-stable protein of 24 kDa,Carhsp1,CRHSP-24,Mouse,Mus musculus
27-882 APOBEC3G is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of th 0.05 mg
31-280 APOBEC3F is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of th 0.05 mg
27-883 Anti-APOBEC3G is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members 0.1 mg
29-678 There are at least four distinct but related alkaline phosphatases intestinal, placental, placental-like, and liver_bone_kidney (tissue non-specific). The first three are located together on chromoso 0.1 mg
DALP-250 QuantiChrom™ Alkaline Phosphatase Assay Kit, Quantitative determination of alkaline phosphatase ALP activity using stable p-nitrophenol phosphate substrate at 405nm 250Tests
27-899 ZNF212 belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, 0.05 mg
27-898 ZNF212 belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur