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Pubmed ID :6302075
Publication Date : //

Low levels of RNase H activity in Escherichia coli FB2 rnh result from a single-base change in the structural gene of RNase H.


The DNA coding for RNase H from a mutant strain of Escherichia coli (FB2) was cloned into plasmid pBR322. DNA sequence analysis and the exchange of a portion of the mutant and wild-type genes revealed that a single-base alteration (C-->T) in the coding region of the structural gene for RNase H is responsible for the difference in RNase H activity of the wild-type and mutant cells.

Authors : Kanaya S , Crouch R J ,

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